Gomes Quintana E
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1985;81(2):461-7. doi: 10.1016/0742-8413(85)90038-6.
A procedure for the isolation of semi-purified membrane fractions containing nicotinic cholinergic receptors from normal and denervated electric organs of the electric eel was developed. A decrease of the binding capacity of [125I]alpha-bungarotoxin was observed on enriched denervated membrane fractions. After denervation, the acetylcholinesterase activity was significantly smaller than that of the normal tissue. Electron microscopic analysis of the different subcellular fractions was performed both for the normal and denervated tissues. The main alteration observed after denervation was a degeneration of the vesicular structures of toxin binding fractions.
已开发出一种从电鳗正常和去神经支配的电器官中分离含有烟碱型胆碱能受体的半纯化膜组分的方法。在富集的去神经支配膜组分上观察到[125I]α-银环蛇毒素结合能力的下降。去神经支配后，乙酰胆碱酯酶活性明显低于正常组织。对正常和去神经支配的组织均进行了不同亚细胞组分的电子显微镜分析。去神经支配后观察到的主要变化是毒素结合组分的囊泡结构退化。
