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Flow cytometric sexing of mammalian sperm.哺乳动物精子的流式细胞术性别鉴定
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A search for sex-specific antigens on bovine spermatozoa using immunological and biochemical techniques to compare the protein profiles of X and Y chromosome-bearing sperm populations separated by fluorescence-activated cell sorting.利用免疫学和生化技术寻找牛精子上的性别特异性抗原，以比较通过荧光激活细胞分选分离出的携带X和Y染色体精子群体的蛋白质谱。
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本文引用的文献

1
Sperm proteome and reproductive technologies in mammals.哺乳动物的精子蛋白质组与生殖技术。
Anim Reprod Sci. 2016 Oct;173:1-7. doi: 10.1016/j.anireprosci.2016.08.008. Epub 2016 Aug 23.
2
Identification and characterization of genes differentially expressed in X and Y sperm using suppression subtractive hybridization and cDNA microarray.利用抑制性消减杂交和cDNA微阵列技术鉴定和表征X精子和Y精子中差异表达的基因。
Mol Reprod Dev. 2014 Oct;81(10):908-17. doi: 10.1002/mrd.22386. Epub 2014 Sep 15.
3
Determination of Sperm Sex Ratio in Bovine Semen Using Multiplex Real-time Polymerase Chain Reaction.利用多重实时聚合酶链反应测定牛精液中的精子性别比例
Asian-Australas J Anim Sci. 2014 Oct;27(10):1411-6. doi: 10.5713/ajas.2014.14223.
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Update on sexed semen technology in cattle.牛性别精液技术的最新进展。
Animal. 2014 May;8 Suppl 1:160-4. doi: 10.1017/S1751731114000202. Epub 2014 Mar 28.
5
Differential protein profile in sexed bovine semen: shotgun proteomics investigation.性别分选牛精液中的差异蛋白质谱：鸟枪法蛋白质组学研究。
Mol Biosyst. 2014 Jun;10(6):1264-71. doi: 10.1039/c3mb70306a. Epub 2013 Nov 13.
6
Tentative identification of sex-specific antibodies and their application for screening bovine sperm proteins for sex-specificity.鉴定性别特异性抗体及其在牛精子蛋白性别特异性筛选中的应用。
Mol Biol Rep. 2014 Jan;41(1):217-23. doi: 10.1007/s11033-013-2854-2. Epub 2013 Nov 12.
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Bovine seminal PDC-109 protein: an overview of biochemical and functional properties.牛精浆PDC - 109蛋白：生化及功能特性概述
Anim Reprod Sci. 2013 Apr;138(1-2):1-13. doi: 10.1016/j.anireprosci.2013.02.008. Epub 2013 Feb 22.
8
Sex selection of sperm in farm animals: status report and developmental prospects.家畜精子的性别选择：现状报告和发展前景。
Reproduction. 2013 Jan 8;145(1):R15-30. doi: 10.1530/REP-12-0151. Print 2013 Jan.
9
Sexing mammalian sperm - Where do we go from here?鉴别哺乳动物精子的性别——我们该何去何从？
J Reprod Dev. 2012;58(5):505-9. doi: 10.1262/jrd.2012-077.
10
Comprehensive mapping of the bull sperm surface proteome.全面绘制公牛精子表面蛋白质组图谱。
Proteomics. 2012 Dec;12(23-24):3559-79. doi: 10.1002/pmic.201200133. Epub 2012 Nov 22.

精子性别鉴定的免疫学方法及鉴定携带X和Y染色体精子的不同方法。

An immunological approach of sperm sexing and different methods for identification of X- and Y-chromosome bearing sperm.

作者信息

Yadav Shiv Kumar, Gangwar Dharmendra Kumar, Singh Jarnail, Tikadar Chiranjeev Kumar, Khanna V Vinoth, Saini Sudha, Dholpuria Sunny, Palta Prabhat, Manik Radhey Shyam, Singh Manoj Kumar, Singla Suresh Kumar

机构信息

Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal - 132 001, Haryana, India.

出版信息

Vet World. 2017 May;10(5):498-504. doi: 10.14202/vetworld.2017.498-504. Epub 2017 May 9.

DOI:10.14202/vetworld.2017.498-504
PMID:28620252
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5465762/
Abstract

Separation of X- and Y-chromosome bearing sperm has been practiced for selection of desired sex of offspring to increase the profit in livestock industries. At present, fluorescence-activated cell sorter is the only successful method for separation of X- and Y-chromosome bearing sperm. This technology is based on the differences in DNA content between these two types of sperm and has been commercialized for bovine sperm. However, this technology still has problems in terms of high economic cost, sperm damage, and lower pregnancy rates compared to unsorted semen. Therefore, an inexpensive, convenient, and non-invasive approach for sperm sexing would be of benefit to agricultural sector. Within this perspective, immunological sperm sexing method is one of the attractive choices to separate X- and Y-chromosome bearing sperm. This article reviews the current knowledge about immunological approaches, viz., H-Y antigen, sex-specific antigens, and differentially expressed proteins for sperm sexing. Moreover, this review also highlighted the different methods for identification of X- and Y-sperm.

摘要

为了提高畜牧业的利润，人们一直在尝试分离携带X和Y染色体的精子以选择后代的理想性别。目前，荧光激活细胞分选仪是分离携带X和Y染色体精子的唯一成功方法。该技术基于这两种精子DNA含量的差异，并且已经用于牛精子的商业化生产。然而，与未分选的精液相比，该技术在经济成本高、精子损伤和妊娠率较低方面仍然存在问题。因此，一种廉价、方便且非侵入性的精子性别鉴定方法将对农业部门有益。从这个角度来看，免疫精子性别鉴定方法是分离携带X和Y染色体精子的有吸引力的选择之一。本文综述了有关免疫方法的现有知识，即用于精子性别鉴定的H-Y抗原、性别特异性抗原和差异表达蛋白。此外，本综述还强调了鉴定X和Y精子的不同方法。