Akhrem A A, Kisel M A, Kozlov I A, Tsybovsky I S, Vulfson E N
FEBS Lett. 1985 Aug 5;187(2):249-52. doi: 10.1016/0014-5793(85)81252-7.
1,5-Difluoro-2,4-dinitrobenzene completely inhibits F1-ATPase when it is used at micromolar concentrations and the inhibitor/enzyme molar ratio is equal to 3. The inhibition can be reversed by dithiothreitol treatment. 7-Chloro-4-nitrobenzofurazan treatment of F1-ATPase does not prevent the reaction of the enzyme with 1,5-difluoro-2,4-dinitrobenzene. The 1,5-difluoro-2,4-dinitrobenzene-induced inhibition is thought to be a result of the modification of a tyrosine residue with pK 9.1.
1,5-二氟-2,4-二硝基苯在微摩尔浓度下使用且抑制剂/酶摩尔比等于3时，能完全抑制F1-ATP酶。这种抑制作用可通过二硫苏糖醇处理逆转。用7-氯-4-硝基苯并呋咱处理F1-ATP酶并不能阻止该酶与1,5-二氟-2,4-二硝基苯的反应。1,5-二氟-2,4-二硝基苯诱导的抑制作用被认为是一个pK为9.1的酪氨酸残基发生修饰的结果。
