Busolini Fabricio Ivan, Rosales Gabriela Judith, Filippa Verónica Palmira, Mohamed Fabian Heber
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 5700 San Luis, Argentina.
Histologia, Departamento de Bioquímica y Ciencias Biológicas, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Avenida Ejercito de los Andes 950, Bloque I, Piso No. 1, San Luis, 5700, Argentina.
Anat Rec (Hoboken). 2017 Oct;300(10):1847-1857. doi: 10.1002/ar.23621. Epub 2017 Jun 27.
The pineal gland of viscacha exhibits histophysiological variations throughout the year, with periods of maximal activity in winter and minimal activity in summer. The aim of this work is to analyze the interstitial cells (IC) in the pineal gland of male viscachas in relation to season and age. The S-100 protein, glio-fibrillary acidic protein (GFAP), and vimentin were detected in adult and immature animals by immunohistochemistry (IHC). Double-IHC was also performed. The S-100 protein was localized within both, IC nucleus and cytoplasm. GFAP was present only in the cytoplasm. Vimentin was expressed in some IC, besides endothelial cells, and perivascular spaces. In the adult males, the morphometric parameters analyzed for the S-100 protein and GFAP exhibited seasonal variations with higher values of immunopositive area percentage in winter and lower values in summer, whereas the immature ones showed the lowest values for all the adult animals studied. Colocalization of S-100 protein and GFAP was observed. The IC exhibited differential expression for the proteins studied, supporting the hypothesis of the neuroectodermal origin. The IC generate an intraglandular communication network, suggesting its participation in the glandular activity regulation processes. The results of double-IHC might indicate the presence of IC in different functional stages, probably related to the needs of the cellular microenvironment. The morphometric variations in the proteins analyzed between immature and adult viscachas probed to be more salient in the latter, suggesting a direct relationship between the expression of the S-100 protein and GFAP, and animal age. Anat Rec, 2017. © 2017 Wiley Periodicals Inc. Anat Rec, 300:1847-1857, 2017. © 2017 Wiley Periodicals, Inc.
毛丝鼠的松果体在全年呈现出组织生理变化，冬季活动最为活跃，夏季活动最少。本研究的目的是分析雄性毛丝鼠松果体中的间质细胞（IC）与季节和年龄的关系。通过免疫组织化学（IHC）在成年和未成年动物中检测S-100蛋白、胶质纤维酸性蛋白（GFAP）和波形蛋白。还进行了双重免疫组织化学。S-100蛋白定位于IC的细胞核和细胞质中。GFAP仅存在于细胞质中。波形蛋白除了在内皮细胞和血管周围间隙中表达外，还在一些IC中表达。在成年雄性中，对S-100蛋白和GFAP分析的形态学参数呈现出季节性变化，冬季免疫阳性面积百分比值较高，夏季较低，而未成年动物在所有研究的成年动物中显示出最低值。观察到S-100蛋白和GFAP的共定位。IC对所研究的蛋白质表现出差异表达，支持神经外胚层起源的假说。IC形成了腺内通讯网络，表明其参与了腺体活动调节过程。双重免疫组织化学的结果可能表明IC处于不同的功能阶段，可能与细胞微环境的需求有关。未成年和成年毛丝鼠之间分析的蛋白质的形态学变化在后者中更为显著，表明S-100蛋白和GFAP的表达与动物年龄之间存在直接关系。《解剖学记录》，2017年。©2017威利期刊公司。《解剖学记录》，300:1847 - 1857，2017年。©2017威利期刊公司。
