Waissi G C, Väänänen K, Nybom I, Pakarinen K, Akkanen J, Leppänen M T, Kukkonen J V K
University of Eastern Finland, Department of Environmental and Biological Sciences, P.O. Box 111, FI-80101 Joensuu, Finland.
Finnish Environment Institute, Survontie 9 A, FI-40500 Jyväskylä, Finland.
Environ Pollut. 2017 Oct;229:423-430. doi: 10.1016/j.envpol.2017.06.015. Epub 2017 Jun 14.
The life cycle parameters of the benthic invertebrate Chironomus riparius make it a relevant organism for use in multi-generation chronic ecotoxicology tests. Since studies on chronic exposures with fullerene carbon nanoparticles have revealed adverse effects at lower concentration ranges, it is crucial to gain understanding of the consequences in following generations. The aims of this study were to investigate whether sediment-associated fullereneC impacts on C. riparius emergence and breeding, thus affecting the growth of the second generation. Larvae were exposed to fullerene-spiked sediment at concentrations of 0.5, 10 and 40 mg/kg sediment dw. Total emergence and breeding success were monitored after the first generation and the newly hatched larvae from the first generation exposure were transferred either to continuous exposure or to pristine sediment without fullerene. Findings indicate that the presence of fullerenes has major impacts on the first generation, mainly shown as delayed emergence time of females. Increased larval growth was observed in the second generation, and we conclude that the C. riparius response to fullerene exposure indicated significant signs of recovery in second-generation larval growth. The result shows the effects to be important for population dynamics, revealing delayed female emergence time, which leads to situation where adults' breeding is impaired.
底栖无脊椎动物摇蚊的生命周期参数使其成为多代慢性生态毒理学测试中的一种相关生物。由于对富勒烯碳纳米颗粒慢性暴露的研究已揭示在较低浓度范围内存在不利影响，因此了解对后代的影响至关重要。本研究的目的是调查与沉积物相关的富勒烯碳是否会影响摇蚊的羽化和繁殖，从而影响第二代的生长。将幼虫暴露于浓度为0.5、10和40毫克/千克干重沉积物的富勒烯强化沉积物中。在第一代之后监测总羽化率和繁殖成功率，并将第一代暴露后新孵化的幼虫转移到持续暴露环境或不含富勒烯的原始沉积物中。研究结果表明，富勒烯的存在对第一代有重大影响，主要表现为雌性羽化时间延迟。在第二代中观察到幼虫生长增加，我们得出结论，摇蚊对富勒烯暴露的反应表明第二代幼虫生长有显著的恢复迹象。结果表明这些影响对种群动态很重要，揭示了雌性羽化时间延迟，这导致成虫繁殖受到损害的情况。
