Department of Microbiology and Immunology, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4B, 036 01, Martin, Slovakia.
Clinical Biochemistry, Joint Stock Company, Department of Clinical Microbiology, University of Zilina, 47A V. Spanyola Street, 010 01, Zilina, Slovakia.
Adv Exp Med Biol. 2017;1021:37-42. doi: 10.1007/5584_2017_26.
Matrix-assisted laser desorption ionization - time-of-flight (MALDI-TOF) mass spectrometry enables to identify microorganisms by comparison of the protein content with reference spectra in the database. The aim of this study was to evaluate the efficacy of phenotypic identification of mycobacteria by MALDI-TOF mass spectrometry in laboratory practice. Seventy five isolates of mycobacteria were identified by molecular and phenotypic method, and the results were compared by MALDI-TOF. For MALDI-TOF, material was processed according to the Bruker Daltonics protocol and Mycobacterial Library database version 2.0, with 313 reference mycobacteria spectra. All except one of the 72 isolates agreed with regard to the species and genus by both methods. Forty three isolates were identified as the M. tuberculosis complex by MALDI-TOF. Thirty one isolates of nontuberculous mycobacteria were consistently identified by both methods to the species level. We conclude that MALDI-TOF mass spectrometry is an accurate method of bacterial identification. Simplicity, speed, and economic availability of the method makes it suitable for mycobacteria identification in a routine laboratory.
基质辅助激光解吸电离 - 飞行时间（MALDI-TOF）质谱法通过将蛋白质含量与数据库中的参考光谱进行比较，从而实现对微生物的鉴定。本研究旨在评估 MALDI-TOF 质谱法在实验室实践中对分枝杆菌表型鉴定的效果。通过分子和表型方法鉴定了 75 株分枝杆菌分离株，并通过 MALDI-TOF 对结果进行了比较。对于 MALDI-TOF，根据 Bruker Daltonics 方案和 Mycobacterial Library 数据库版本 2.0 对材料进行了处理，其中包含 313 个参考分枝杆菌光谱。除了一个分离株之外，所有 72 个分离株在两种方法下均在物种和属上达成一致。通过 MALDI-TOF 鉴定了 43 株结核分枝杆菌复合体。通过两种方法一致鉴定了 31 株非结核分枝杆菌。我们得出结论，MALDI-TOF 质谱法是一种准确的细菌鉴定方法。该方法简单、快速且经济适用，使其适合于常规实验室中的分枝杆菌鉴定。
