School of Pharmacy, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, United Kingdom; Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom.
Univ. Grenoble Alpes - Laboratoire TIMC/IMAG UMR CNRS 5525, Pavillon Taillefer Domaine de la merci, 38700 La Tronche, France.
J Colloid Interface Sci. 2017 Oct 15;504:741-750. doi: 10.1016/j.jcis.2017.05.086. Epub 2017 May 29.
It is known that nanoparticles (NPs) in a biological fluid are immediately coated by a protein corona (PC), composed of a hard (strongly bounded) and a soft (loosely associated) layers, which represents the real nano-interface interacting with the cellular membrane in vivo. In this regard, supported lipid bilayers (SLB) have extensively been used as relevant model systems for elucidating the interaction between biomembranes and NPs. Herein we show how the presence of a PC on the NP surface changes the interaction between NPs and lipid bilayers with particular care on the effects induced by the NPs on the bilayer structure.
In the present work we combined Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D) and Neutron Reflectometry (NR) experimental techniques to elucidate how the NP-membrane interaction is modulated by the presence of proteins in the environment and their effect on the lipid bilayer.
Our study showed that the NP-membrane interaction is significantly affected by the presence of proteins and in particular we observed an important role of the soft corona in this phenomenon.
众所周知，生物流体中的纳米颗粒（NPs）会立即被蛋白质冠（PC）覆盖，PC 由硬（强结合）和软（弱结合）层组成，它代表了与体内细胞膜相互作用的真实纳米界面。在这方面，支持脂质双层（SLB）已被广泛用作阐明生物膜与 NPs 之间相互作用的相关模型系统。本文展示了 NP 表面 PC 的存在如何改变 NPs 与脂质双层之间的相互作用，特别关注 NPs 对双层结构诱导的影响。
在本工作中，我们结合了石英晶体微天平与耗散监测（QCM-D）和中子反射率（NR）实验技术，以阐明环境中蛋白质的存在如何调节 NP-膜相互作用及其对脂质双层的影响。
我们的研究表明，NP-膜相互作用受到蛋白质存在的显著影响，特别是我们观察到软冠在这一现象中起着重要作用。
