Zhang Kui, Pan Guangzhao, Zhao Yuzu, Hao Xiangwei, Li Chongyang, Shen Li, Zhang Rui, Su Jingjing, Cui Hongjuan
State Key Laboratory of Silkworm Genome Biology, The Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400716, China.
State Key Laboratory of Silkworm Genome Biology, The Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400716, China.
Mol Immunol. 2017 Aug;88:106-115. doi: 10.1016/j.molimm.2017.06.023. Epub 2017 Jun 14.
Insects have evolved an effective immune system to respond to various challenges. In this study, a novel immune-related gene, called BmHDD1, was first charactered in silkworm, Bombyx mori. BmHDD1 contained an ORF of 837bp and encoding a deduced protein of 278 amino acids. BmHDD1 was specifically expressed in hemocytes, and highly expressed at the molting and metamorphosis stages under normal physiological conditions. Our results suggested that BmHDD1 was mainly generated by hemocytes and secreted into hemolymph. Our results also showed that the expression level of BmHDD1 was significantly increased after 20E injection, which indicated that BmHDD1 might be regulated by ecdysone. More importantly, BmHDD1 was dramatically induced after injected with different types of PAMPs or bacteria, either in hemocytes or fat body. Those results suggested that BmHDD1 plays a role in developing and immunity system in silkworm, Bombyx mori.
昆虫已经进化出一种有效的免疫系统来应对各种挑战。在本研究中，一个名为BmHDD1的新型免疫相关基因首次在家蚕中得到鉴定。BmHDD1含有一个837bp的开放阅读框，编码一个由278个氨基酸组成的推导蛋白。BmHDD1在家蚕血细胞中特异性表达，在正常生理条件下的蜕皮和变态阶段高度表达。我们的结果表明，BmHDD1主要由血细胞产生并分泌到血淋巴中。我们的结果还表明，注射20E后BmHDD1的表达水平显著增加，这表明BmHDD1可能受蜕皮激素调节。更重要的是，在注射不同类型的病原体相关分子模式或细菌后，无论是在血细胞还是脂肪体中，BmHDD1都被显著诱导。这些结果表明，BmHDD1在家蚕的发育和免疫系统中发挥作用。
