Wu Wei, Xiong Wenfeng, Li Chengjun, Zhai Mengfan, Li Yao, Ma Fei, Li Bin
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.
Genomics. 2017 Oct;109(5-6):362-373. doi: 10.1016/j.ygeno.2017.06.001. Epub 2017 Jun 15.
To date, although some microRNAs (miRNAs) have been discovered in the holometabolism insect Tribolium castaneum, large numbers of miRNAs still require investigation. Knocking down Dicer-1 (Dcr-1) and Argonaute-1 (Ago-1) in late larvae impaired miRNA synthesis, affected the juvenile hormone pathway by up-regulating Methoprene-tolerant (Met) and Krüppel-homolog1 (Kr-h1) transcript levels, and resulted in a series of defects in T. castaneum development and metamorphosis. Thus, high-throughput Illumina/Solexa sequencing was performed with a mixed sample of eight key developmental stages of T. castaneum. In total, 1154 unique miRNAs were discovered containing 274 conserved miRNAs belong to 68 miRNA families, 108 known candidate miRNAs and 772 novel miRNAs. Genome locus analysis showed that miRNA clusters are more abundant in T. castaneum than other species. The results indicated that RNAi of Dcr-1 and Ago-1 in T. castaneum resulted in miRNA-induced metamorphosis defects. Furthermore, large numbers of novel miRNAs were discovered in T. castaneum and localized to T. castaneum genome loci.
迄今为止，尽管在全变态昆虫赤拟谷盗中已经发现了一些微小RNA（miRNA），但仍有大量miRNA有待研究。在晚期幼虫中敲低Dicer-1（Dcr-1）和Argonaute-1（Ago-1）会损害miRNA合成，通过上调耐甲氧普烯（Met）和Krüppel同源物1（Kr-h1）的转录水平影响保幼激素途径，并导致赤拟谷盗发育和变态出现一系列缺陷。因此，对赤拟谷盗八个关键发育阶段的混合样本进行了高通量Illumina/Solexa测序。总共发现了1154个独特的miRNA，其中包括属于68个miRNA家族的274个保守miRNA、108个已知的候选miRNA和772个新miRNA。基因组位点分析表明，赤拟谷盗中的miRNA簇比其他物种更为丰富。结果表明，赤拟谷盗中Dcr-1和Ago-1的RNA干扰导致了miRNA诱导的变态缺陷。此外，在赤拟谷盗中发现了大量新miRNA，并定位到赤拟谷盗基因组位点。
