BioMedical Center, Ludwig Maximilians University, Großhaderner Str. 9, Planegg-Martinsried 82152, Germany.
BioMedical Center, Ludwig Maximilians University, Großhaderner Str. 9, Planegg-Martinsried 82152, Germany.
Methods. 2017 Aug 15;126:177-185. doi: 10.1016/j.ymeth.2017.06.013. Epub 2017 Jun 15.
In polarized cells, such as neurons, the synthesis of an mRNA does not ensure its proper cellular expression. Most mature transcripts require the association with RNA-binding proteins, resulting in the formation of RNA granules, which are then transported within the cytoplasm along the cytoskeleton and delivered to their proper subcellular locations, where they can be locally translated. Here we review current microscopy methods that have been developed to visualize RNA granule formation, transport and translation at the single cell level with a special emphasis on the MS2 and SunTag systems. They include the labeling of mRNAs and RNA-binding proteins in living cells or even the detection of newly synthesized proteins in situ.
在极化细胞（如神经元）中，mRNA 的合成并不能确保其在细胞内的正确表达。大多数成熟的转录本需要与 RNA 结合蛋白结合，从而形成 RNA 颗粒，这些颗粒随后沿着细胞骨架在细胞质内运输，并被递送到其适当的亚细胞位置，在那里可以进行局部翻译。在这里，我们综述了目前已开发的显微镜方法，这些方法可用于在单细胞水平上可视化 RNA 颗粒的形成、运输和翻译，特别强调了 MS2 和 SunTag 系统。它们包括在活细胞中标记 mRNA 和 RNA 结合蛋白，甚至原位检测新合成的蛋白质。
