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磁铁矿葡聚糖-精胺纳米颗粒在乳腺癌热疗中的合成与应用

Synthesis and application of magnetite dextran-spermine nanoparticles in breast cancer hyperthermia.

作者信息

Avazzadeh Reza, Vasheghani-Farahani Ebrahim, Soleimani Masoud, Amanpour Saeid, Sadeghi Mohsen

机构信息

Biomedical Engineering Group, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.

Hematology Group, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

出版信息

Prog Biomater. 2017 Sep;6(3):75-84. doi: 10.1007/s40204-017-0068-8. Epub 2017 Jun 17.

DOI:10.1007/s40204-017-0068-8
PMID:28624871
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5597569/
Abstract

Cancer treatment has been very challenging in recent decades. One of the most promising cancer treatment methods is hyperthermia, which increases the tumor temperature (41-45 °C). Magnetic nanoparticles have been widely used for selective targeting of cancer cells. In the present study, magnetic dextran-spermine nanoparticles, conjugated with Anti-HER2 antibody to target breast cancer cells were developed. The magnetic dextran-spermine nanoparticles (DMNPs) were prepared by ionic gelation, followed by conjugation of antibody to them using EDC-NHS method. Then the Prussian blue method was used to estimate the targeting ability and cellular uptake. Cytotoxicity assay by MTT showed that antibody-conjugated MNPs (ADMNPs) have no toxic effect on SKBR3 and human fibroblast cells. Finally, the hyperthermia was applied to show that synthesized ADMNPs, could increase the cancer cells temperature up to 45 °C and kill most of them without affecting normal cells. These observations proved that Anti-HER2 conjugated magnetic dextran-spermine nanoparticles can target and destroy cancer cells and are potentially suitable for cancer treatment.

摘要

近几十年来，癌症治疗一直极具挑战性。最有前景的癌症治疗方法之一是热疗，即提高肿瘤温度（41-45°C）。磁性纳米颗粒已被广泛用于癌细胞的选择性靶向。在本研究中，开发了与抗HER2抗体偶联以靶向乳腺癌细胞的磁性葡聚糖-精胺纳米颗粒。磁性葡聚糖-精胺纳米颗粒（DMNPs）通过离子凝胶法制备，然后使用EDC-NHS方法将抗体偶联到其上。然后使用普鲁士蓝法评估靶向能力和细胞摄取。MTT细胞毒性试验表明，抗体偶联的磁性纳米颗粒（ADMNPs）对SKBR3和人成纤维细胞没有毒性作用。最后，应用热疗表明合成的ADMNPs可以将癌细胞温度提高到45°C并杀死大多数癌细胞，而不影响正常细胞。这些观察结果证明，抗HER2偶联的磁性葡聚糖-精胺纳米颗粒可以靶向并破坏癌细胞，并且可能适用于癌症治疗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d411/5597569/844c801bf454/40204_2017_68_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d411/5597569/cf32504c5272/40204_2017_68_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d411/5597569/bfdc953b07bd/40204_2017_68_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d411/5597569/d772da7f095b/40204_2017_68_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d411/5597569/43c9f095049a/40204_2017_68_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d411/5597569/b28b014cad4c/40204_2017_68_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d411/5597569/9680e0047530/40204_2017_68_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d411/5597569/844c801bf454/40204_2017_68_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d411/5597569/cf32504c5272/40204_2017_68_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d411/5597569/bfdc953b07bd/40204_2017_68_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d411/5597569/d772da7f095b/40204_2017_68_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d411/5597569/43c9f095049a/40204_2017_68_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d411/5597569/b28b014cad4c/40204_2017_68_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d411/5597569/9680e0047530/40204_2017_68_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d411/5597569/844c801bf454/40204_2017_68_Fig7_HTML.jpg

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本文引用的文献

[1]
Toxicity evaluation of magnetic hyperthermia induced by remote actuation of magnetic nanoparticles in 3D micrometastasic tumor tissue analogs for triple negative breast cancer.

Biomaterials. 2017-3

[2]
Thermal analysis of magnetic nanoparticle in alternating magnetic field on human HCT-116 colon cancer cell line.

Int J Hyperthermia. 2016-12

[3]
Functionalized magnetic dextran-spermine nanocarriers for targeted delivery of doxorubicin to breast cancer cells.

Int J Pharm. 2016-3-30

[4]
Efficient treatment of breast cancer xenografts with multifunctionalized iron oxide nanoparticles combining magnetic hyperthermia and anti-cancer drug delivery.

Breast Cancer Res. 2015-5-13

[5]
Carbon encapsulated iron oxide nanoparticles surface engineered with polyethylene glycol-folic acid to induce selective hyperthermia in folate over expressed cancer cells.

Int J Pharm. 2015-1-16

[6]
Cylindrical agar gel with fluid flow subjected to an alternating magnetic field during hyperthermia.

Int J Hyperthermia. 2015-2

[7]
PEGylated Prussian blue nanocubes as a theranostic agent for simultaneous cancer imaging and photothermal therapy.

Biomaterials. 2014-9-16

[8]
Perspectives of breast cancer thermotherapies.

J Cancer. 2014-5-29

[9]
Thermally responsive nanoparticle-encapsulated curcumin and its combination with mild hyperthermia for enhanced cancer cell destruction.

Acta Biomater. 2014-2

[10]
Synthesis and characterization of CREKA-conjugated iron oxide nanoparticles for hyperthermia applications.

Acta Biomater. 2014-1-31

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