Institute of Functional Nano & Soft Materials Laboratory (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, Jiangsu 215123, China.
Institute of Functional Nano & Soft Materials Laboratory (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, Jiangsu 215123, China.
Biomaterials. 2014 Dec;35(37):9844-9852. doi: 10.1016/j.biomaterials.2014.09.004. Epub 2014 Sep 16.
Theranostic agents with both imaging and therapeutic functions have attracted enormous interests in cancer diagnosis and treatment in recent years. In this work, we develop a novel theranostic agent based on Prussian blue nanocubes (PB NCs), a clinically approved agent with strong near-infrared (NIR) absorbance and intrinsic paramagnetic property, for in vivo bimodal imaging-guided photothermal therapy. After being coated with polyethylene glycol (PEG), the obtained PB-PEG NCs are highly stable in various physiological solutions. In vivo T1-weighted magnetic resonance (MR) and photoacoustic tomography (PAT) bimodal imaging uncover that PB-PEG NCs after intravenous (i.v.) injection show high uptake in the tumor. Utilizing the strong and super stable NIR absorbance of PB, in vivo cancer treatment is then conducted upon i.v. injection of PB-PEG NCs followed by NIR laser irradiation of the tumors, achieving excellent therapeutic efficacy in a mouse tumor model. Comprehensive blood tests and careful histological examinations reveal no apparent toxicity of PB-PEG NCs to mice at our tested dose, which is two-fold of the imaging/therapy dose, within two months. Our work highlights the great promise of Prussian blue with well engineered surface coating as a multifunctional nanoprobe for imaging-guided cancer therapy.
近年来，集成像和治疗功能于一体的诊疗试剂在癌症的诊断和治疗方面引起了广泛关注。在这项工作中，我们基于普鲁士蓝纳米立方（PB NCs）开发了一种新型诊疗试剂，该试剂已被临床批准，具有很强的近红外（NIR）吸收和固有顺磁性，可用于体内双模成像引导光热治疗。用聚乙二醇（PEG）对其进行修饰后，得到的 PB-PEG NCs 在各种生理溶液中具有很高的稳定性。体内 T1 加权磁共振（MR）和光声断层扫描（PAT）双模成像显示，静脉注射（i.v.）后 PB-PEG NCs 在肿瘤部位有很高的摄取。利用 PB 强而稳定的近红外吸收，静脉注射 PB-PEG NCs 后再用近红外激光照射肿瘤，可在小鼠肿瘤模型中实现优异的治疗效果。全面的血液测试和仔细的组织学检查表明，在我们测试的剂量下（两倍于成像/治疗剂量），两个月内 PB-PEG NCs 对小鼠没有明显的毒性。我们的工作突出了经过精心设计的表面涂层普鲁士蓝作为一种多功能纳米探针，在成像引导癌症治疗方面具有广阔的应用前景。
