LY294002，一种 PI3K 抑制剂，可减轻大鼠的抽动秽语综合征。
LY294002, a PI3K inhibitor, attenuates Tourette syndrome in rats.
机构信息
Central Laboratory, Nanjing Municipal Hospital of T.C.M., The Third Affiliated Hospital of Nanjing University of T.C.M., Nanjing, 210001, China.
Nanjing University of T.C.M., Nanjing, 210046, China.
出版信息
Metab Brain Dis. 2017 Oct;32(5):1619-1625. doi: 10.1007/s11011-017-0051-z. Epub 2017 Jun 18.
The present study was designed to investigate the effects of LY294002 on Tourette syndrome (TS) in rats. TS model was induced in rats by DOI (the selective 5-HT2A/2C agonist 1- (2, 5- dimethoxy -4 - iodophenyl) -2- aminopropane). Behavior was assessed by stereotypic score and autonomic activity. Inflammatory cytokines such as interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in serum and striatum were detected. The protein levels of PI3K/Akt/NF-B in striatum were detected by Western Blot. LY294002 treatment significantly reduced IL-6, IL-1β and TNF-α in serum and striatum of TS rats, Also, highly expressed P-PI3K, P-Akt, P-NF-κBp65, P-IκBα in TS rats were restored respectively by LY294002 treatment as indicted in western blot analysis and immunohistochemistry analysis. Thus, it was supposed that the protective effect of LY294002 against TS in rat might be associated with the regulation of PI3K/Akt/NF-B pathway.
本研究旨在探讨 LY294002 对大鼠抽动秽语综合征（TS）的影响。通过 DOI（5-羟色胺 2A/2C 受体选择性激动剂 1-（2,5-二甲氧基-4-碘苯基）-2-氨基丙烷）诱导大鼠 TS 模型。通过刻板评分和自主活动评估行为。检测血清和纹状体中白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）等炎症细胞因子。通过 Western Blot 检测纹状体中 PI3K/Akt/NF-B 的蛋白水平。LY294002 治疗可显著降低 TS 大鼠血清和纹状体中 IL-6、IL-1β和 TNF-α的表达，Western blot 分析和免疫组织化学分析显示，LY294002 治疗还可分别恢复 TS 大鼠中高表达的 P-PI3K、P-Akt、P-NF-κBp65 和 P-IκBα。因此，推测 LY294002 对大鼠 TS 的保护作用可能与调节 PI3K/Akt/NF-B 通路有关。
Zotero 插件