Zhang Ling, Lin Lin, Song Yu-Peng, Pan Bo, Yang Qing-Hua, Jiang Hai-Yue
Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100144, China.
Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100144, China.
Gene Expr Patterns. 2017 Nov;25-26:131-141. doi: 10.1016/j.gep.2017.06.007. Epub 2017 Jun 15.
To analyse lncRNA expression profiles in microtia using bioinformatics analysis.
We examined lncRNA expression profiles in residual ear cartilage and normal ear cartilage from individual congenital microtia patients.
The gene chips used in this study included 30586 lncRNAs and 26109 mRNA probes. Intotal, 180 lncRNAs with differential expression weredetected in the residual ear cartilage compared with the normal cartilage, including 74 up-regulated and 106down-regulated lncRNAs. Signalling pathway analysis highlighted glyceride metabolism, osteoclast differentiation, andtumour growth. The results of qRT-PCR analysis were consistent with those of themicroarray.
Differential expression of lncRNAs occurs in microtia. These lncRNAs and related signalling pathways may play an important role in the occurrence and development ofmicrotia.
运用生物信息学分析方法，分析小耳畸形中长链非编码RNA（lncRNA）的表达谱。
我们检测了先天性小耳畸形患者残留耳软骨和正常耳软骨中的lncRNA表达谱。
本研究使用的基因芯片包含30586个lncRNA和26109个mRNA探针。与正常软骨相比，在残留耳软骨中共检测到180个差异表达的lncRNA，其中74个lncRNA上调，106个lncRNA下调。信号通路分析显示甘油酯代谢、破骨细胞分化和肿瘤生长相关通路。定量逆转录聚合酶链反应（qRT-PCR）分析结果与基因芯片结果一致。
lncRNA在小耳畸形中存在差异表达。这些lncRNA及相关信号通路可能在小耳畸形的发生发展中起重要作用。
