Pei Lin-Guo, Yuan Chao, Guo Yi-Tian, Kou Hao, Xia Li-Ping, Zhang Li, Yan You-E, Xu Dan, Wang Hui
Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan, China; Basic Medical College of Nanyang Medical University, Nanyang 473061, China.
Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan, China.
Reprod Toxicol. 2017 Aug;71:150-158. doi: 10.1016/j.reprotox.2017.06.045. Epub 2017 Jun 15.
Our previous studies have demonstrated that prenatal caffeine exposure (PCE) induced an intrauterine programming of hypothalamic-pituitary-adrenal axis (HPAA)-associated neuroendocrine metabolism in 3-month-old offspring rats. In this study, we aimed to confirm this programming disorder and high susceptibility to metabolic syndrome (MS) in 10-month-old female PCE offspring with postnatal catch-up growth. We found that PCE female offspring rats showed decreased bodyweight but a higher rate of weight gain after birth. Moreover, in the offspring, basal hyperinsulinemia and insulin resistance were observed before unpredictable chronic stress (UCS), but serum total cholesterol (TCH) levels and triglyceride/high-density lipoprotein-cholesterol (TG/HDL-C), TCH/HDL-C and low-density lipoprotein-cholesterol/HDL-C (LDL-C/HDL-C) ratio changes were increased after UCS, accompanied by morphological damage of the related tissues. These results suggested that PCE adult female offspring rats were highly susceptible to MS, which is related to HPAA-associated neuroendocrine-metabolic programming disorder.
我们之前的研究表明，孕期咖啡因暴露（PCE）会导致3月龄子代大鼠下丘脑-垂体-肾上腺轴（HPAA）相关神经内分泌代谢的宫内编程。在本研究中，我们旨在证实这种编程紊乱以及产后追赶生长的10月龄PCE雌性子代对代谢综合征（MS）的高易感性。我们发现，PCE雌性子代大鼠出生时体重下降，但出生后体重增加率更高。此外，在子代中，在不可预测的慢性应激（UCS）之前观察到基础高胰岛素血症和胰岛素抵抗，但UCS后血清总胆固醇（TCH）水平以及甘油三酯/高密度脂蛋白胆固醇（TG/HDL-C）、TCH/HDL-C和低密度脂蛋白胆固醇/高密度脂蛋白胆固醇（LDL-C/HDL-C）比值升高，同时伴有相关组织的形态学损伤。这些结果表明，PCE成年雌性子代大鼠对MS高度易感，这与HPAA相关神经内分泌代谢编程紊乱有关。
