Assessment of radicular dentin permeability after irradiation with CO laser and endodontic irrigation treatments with thermal imaging.

Previous studies have demonstrated that the permeability changes due to the surface modification of dentin can be quantified via thermal imaging during dehydration. The CO laser has been shown to remove the smear layer and disinfect root canals. Moreover, thermal modification via CO laser irradiation can be used to convert dentin into a highly mineralized enamel-like mineral. The purpose of this study is to evaluate the radicular dentin surface modification after CO laser irradiation by measuring the permeability with thermal imaging. Human molar specimens (n=12) were sectioned into 4 axial walls of the pulp chamber and treated with either 10% NaClO for 1 minute, 5% EDTA for 1 minute, CO laser or none. The CO laser was operated at 9.4 μm with a pulse duration of 26 μs, pulse repetition rate of 300 Hz and a fluence of 13 J/cm. The samples were dehydrated using an air spray for 60 seconds and imaged using a thermal camera. The resulting surface morphological changes were assessed using 3D digital microscopy. The images from digital microscopy confirmed melting of the mineral phase of dentin. The area enclosed by the time-temperature curve during dehydration, ΔQ, measured with thermal imaging increased significantly with treatments with EDTA and the CO laser (P<0.05). These results indicate that the surface modification due to CO laser treatment increases permeability of radicular dentin.