Feng Gang, Huang Hou-Bao, Ye Xiao-Bing, Zhang Peng, Huang Jian-Jun, Huang Li-Zhu, Cheng Long, Pu Chun, Li Guorong
Clinical Laboratory, The First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui 241001, China.
Department of Urology, The First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui 241001, China.
Dis Markers. 2017;2017:2543059. doi: 10.1155/2017/2543059. Epub 2017 May 24.
CD146 is a membrane signal receptor in tumor-induced angiogenesis. However, limited studies have focused on the CD146 promoter polymorphisms in clear cell renal cell carcinoma (ccRCC).
The purpose of this study was to investigate the association between polymorphisms located in the promoter region of the CD146 gene and characteristics of ccRCC in Chinese population. The association between the CD146 promoter polymorphisms and CD146 expression was also investigated in ccRCC.
A total of 600 samples including 300 ccRCC patients and 300 healthy controls were collected for analysis of the CD146 promoter polymorphisms by direct sequence. The CD146 expressions were measured by qRT-PCR.
We had not found any significant differences in genotypic and allelic frequencies of CD146 promoter polymorphisms between ccRCC patients and controls. The rs3923594 was associated with stage and metastasis (300 cases) and recurrence (263 cases) of ccRCC in Chinese population. A significant association was also observed between the rs3923594 and CD146 expression (227 cases) in ccRCC.
CD146 promoter polymorphisms were not associated with the risk of ccRCC in Chinese population. The rs3923594 was an independent predictor of recurrence in Chinese patients with localized ccRCC.
CD146是肿瘤诱导血管生成中的一种膜信号受体。然而，针对透明细胞肾细胞癌（ccRCC）中CD146启动子多态性的研究有限。
本研究旨在探讨中国人群中CD146基因启动子区域多态性与ccRCC特征之间的关联。同时也研究了ccRCC中CD146启动子多态性与CD146表达之间的关联。
共收集600份样本，包括300例ccRCC患者和300例健康对照，通过直接测序分析CD146启动子多态性。采用qRT-PCR检测CD146表达。
我们未发现ccRCC患者与对照之间CD146启动子多态性的基因型和等位基因频率存在任何显著差异。rs3923594与中国人群ccRCC的分期和转移（300例）及复发（263例）相关。在ccRCC中，rs3923594与CD146表达（227例）之间也观察到显著关联。
在中国人群中，CD146启动子多态性与ccRCC风险无关。rs3923594是中国局限性ccRCC患者复发的独立预测因子。
