Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University , Shenyang, Liaoning 110016, People's Republic of China.
J Agric Food Chem. 2017 Jul 12;65(27):5570-5580. doi: 10.1021/acs.jafc.7b02053. Epub 2017 Jun 28.
The main polyphenols in mung bean (Vigna radiata L.) seed (MBS), an edible legume with various biological activities, are C-glycosyl flavones (vitexin, isovitexin, isovitexin-6″-O-α-l-glucoside, and dulcinoside). In our study, a validated ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed to quantitate the concentrations of four C-glycosyl flavones from MBS extracts in the plasma and various tissues of rats and successfully applied to study their pharmacological profile and tissue distribution in vivo. Four C-glycosyl flavones were rapidly absorbed after oral administration, achieving a C at around 1.5 h, and they could be distributed widely and rapidly in tested tissues. The concentrations of four C-glycosyl flavones in all of the tested tissues decreased obviously in 4 h, which indicated that there was not a trend of long-term accumulation of them. This is the first time to report on pharmacokinetic and tissue distribution studies of four C-glycosyl flavones in rat. The results provided a significative basis for the application of MBS.
绿豆（Vigna radiata L.）种子中的主要多酚类物质是 C-糖基黄酮（牡荆素、异牡荆素、异牡荆素-6”-O-α-l-葡萄糖苷和甜菊糖苷），它们是一种具有多种生物活性的食用豆类。在我们的研究中，建立了一种经过验证的超高效液相色谱-串联质谱法（UHPLC-MS/MS），用于定量测定绿豆提取物中四种 C-糖基黄酮在大鼠血浆和各种组织中的浓度，并成功应用于研究它们在体内的药理学特征和组织分布。四种 C-糖基黄酮经口服给药后迅速吸收，C 达峰时间约为 1.5 小时，它们可以在测试的组织中广泛而迅速地分布。四种 C-糖基黄酮在所有测试组织中的浓度在 4 小时内明显下降，这表明它们没有长期积累的趋势。这是首次报道四种 C-糖基黄酮在大鼠体内的药代动力学和组织分布研究。研究结果为绿豆的应用提供了有意义的依据。
