Shen Xin, Liu Xue, Li Rongye, Yun Peng, Li Chenglong, Su Feng, Li Suming
a School of Polymer Science and Engineering, Qingdao University of Science and Technology , Qingdao , China.
b College of Chemical Engineering, Qingdao University of Science and Technology , Qingdao , China.
J Biomater Sci Polym Ed. 2017 Oct;28(15):1677-1694. doi: 10.1080/09205063.2017.1344383. Epub 2017 Jun 22.
A series of poly(ethylene glycol)-polylactide (PEG-PLA) diblock copolymers were synthesized by ring-opening polymerization of l-lactide using monomethoxy PEG as macroinitiator and zinc lactate as catalyst. Filomicelles were prepared from the resulting copolymers by co-solvent evaporation method. The biocompatibility of the various filomicelles was evaluated with the aim of assessing their potential as drug carriers. Various aspects of biocompatibility were considered, including agar diffusion test, MTT assay, release of cytokines, hemolytic test, dynamic clotting time, protein adsorption in vitro, and zebrafish embryonic compatibility in vivo. The results revealed that the filomicelles present good cytocompatibility and hemocompatibility in vitro. Moreover, the cumulative effects of filomicelles throughout embryos deveploping stages have no toxicity in vivo. It is thus concluded that filomicelles prepared from PEG-PLA copolymers with outstanding biocompatibility are promising as potential drug carrier.
以单甲氧基聚乙二醇为大分子引发剂、乳酸锌为催化剂，通过丙交酯的开环聚合反应合成了一系列聚（乙二醇）-聚丙交酯（PEG-PLA）二嵌段共聚物。采用共溶剂蒸发法由所得共聚物制备丝状胶束。为了评估各种丝状胶束作为药物载体的潜力，对其生物相容性进行了评估。考虑了生物相容性的各个方面，包括琼脂扩散试验、MTT 法、细胞因子释放、溶血试验、动态凝血时间、体外蛋白质吸附以及体内斑马鱼胚胎相容性。结果表明，丝状胶束在体外具有良好的细胞相容性和血液相容性。此外，丝状胶束在胚胎发育各阶段的累积效应在体内无毒性。因此得出结论，由具有优异生物相容性的 PEG-PLA 共聚物制备的丝状胶束有望成为潜在的药物载体。
