Damjanov I, Damjanov N, Knowles B B, Engvall E
Virchows Arch B Cell Pathol Incl Mol Pathol. 1985;49(1):45-52. doi: 10.1007/BF02912083.
Monoclonal antibodies reacting exclusively with laminin of human origin and a polyclonal antibody reacting with both murine and human laminin were used to immunohistochemically study the extracellular matrix of four human tumors grown as xenografts in nude mice: a lung carcinoma and a yolk sac carcinoma because they produced cell associated laminin in vitro; and two hepatocellular carcinomas which did not produce cell associated laminin in vitro. The extracellular matrix of the xenografts of the lung carcinoma and the yolk sac carcinoma contained laminin of both human and murine origin. Xenografts of liver carcinoma contained only laminin of mouse origin. This shows that the malignant cells capable of laminin production in vitro contribute this glycoprotein to the extracellular matrix of the solid tumor formed by them in vivo.
使用仅与人源层粘连蛋白反应的单克隆抗体和与鼠源及人源层粘连蛋白均反应的多克隆抗体，对在裸鼠体内作为异种移植生长的四种人类肿瘤的细胞外基质进行免疫组织化学研究：一种肺癌和一种卵黄囊癌，因为它们在体外产生与细胞相关的层粘连蛋白；以及两种在体外不产生与细胞相关层粘连蛋白的肝细胞癌。肺癌和卵黄囊癌异种移植的细胞外基质包含人源和鼠源的层粘连蛋白。肝癌异种移植仅包含鼠源层粘连蛋白。这表明在体外能够产生层粘连蛋白的恶性细胞将这种糖蛋白贡献给它们在体内形成的实体瘤的细胞外基质。
