College of Animal Science and Technology, National Beef Cattle Improvement Center, Northwest A&F University, Yangling Shaanxi 712100, China.
College of Animal Science and Technology, National Beef Cattle Improvement Center, Northwest A&F University, Yangling Shaanxi 712100, China; Key Laboratory of Zoology in Hunan Higher Education, College of Life Science, Hunan University of Arts and Science, Changde Hunan 415000, China.
Mol Cell Probes. 2017 Oct;35:27-33. doi: 10.1016/j.mcp.2017.06.002. Epub 2017 Jun 13.
In human, microRNA-214 (miR-214) plays crucial roles in mechanisms of immunity. However, the potential importance of miR-214 in immune mechanisms in dairy cows has not been investigated. In this study, we assessed potential immunity-related functions of miR-214 in human 293A cells and in bovine mammary epithelial cells (BMECs). We found that NFATc3 and TRAF3 could be targeted by miR-214 in both 293A cells and BMECs. We also found that miR-214 indirectly inhibited the expression of MAP3K14, TBK1 and inflammatory cytokines IL-6 and IL-1β. Taken together, our data revealed miR-214 regulated immunity-related genes by targeting NFATc3 and TRAF3, which provides insight into the molecular basis of immunity.
在人类中，microRNA-214（miR-214）在免疫机制中发挥着关键作用。然而，miR-214 在奶牛免疫机制中的潜在重要性尚未得到研究。在这项研究中，我们评估了 miR-214 在人类 293A 细胞和牛乳腺上皮细胞（BMEC）中的潜在免疫相关功能。我们发现 NFATc3 和 TRAF3 可以作为 miR-214 在 293A 细胞和 BMEC 中的靶点。我们还发现，miR-214 间接抑制了 MAP3K14、TBK1 和炎症细胞因子 IL-6 和 IL-1β 的表达。综上所述，我们的数据揭示了 miR-214 通过靶向 NFATc3 和 TRAF3 来调节免疫相关基因，这为免疫的分子基础提供了新的见解。
