Cóndor M, Steinwachs J, Mark C, García-Aznar J M, Fabry B
Department of Mechanical Engineering, University of Zaragoza, Zaragoza, Spain.
Department of Physics, University of Erlangen-Nuremberg, Erlangen, Germany.
Curr Protoc Cell Biol. 2017 Jun 19;75:10.22.1-10.22.20. doi: 10.1002/cpcb.24.
Cell migration through a three-dimensional (3-D) matrix depends strongly on the ability of cells to generate traction forces. To overcome the steric hindrance of the matrix, cells need to generate sufficiently high traction forces but also need to distribute these forces spatially in a migration-promoting way. This unit describes a protocol to measure spatial maps of cell traction forces in 3-D biopolymer networks such as collagen, fibrin, or Matrigel. Traction forces are computed from the relationship between measured force-induced matrix deformations surrounding the cell and the known mechanical properties of the matrix. The method does not rely on knowledge of the cell surface coordinates and takes nonlinear mechanical properties of the matrix into account. © 2017 by John Wiley & Sons, Inc.
细胞通过三维（3-D）基质的迁移很大程度上取决于细胞产生牵引力的能力。为了克服基质的空间位阻，细胞需要产生足够高的牵引力，并且还需要以促进迁移的方式在空间上分布这些力。本单元描述了一种用于测量三维生物聚合物网络（如胶原蛋白、纤维蛋白或基质胶）中细胞牵引力空间图的方案。牵引力是根据围绕细胞测量的力诱导基质变形与基质已知力学性能之间的关系计算得出的。该方法不依赖于细胞表面坐标的知识，并考虑了基质的非线性力学性能。© 2017约翰威立国际出版公司。
