Wolter H J
Biochem Biophys Res Commun. 1985 Jul 31;130(2):774-80. doi: 10.1016/0006-291x(85)90483-8.
Adjacent serial sections through the rat duodenum were alternately stained for immunofluorescence microscopic studies with specific anti-sera directed to the opioid peptides dynorphin-A(1-17) and dynorphin-A(1-8), respectively. This resulted in the evidence that two separate dynorphinergic neuron populations are present there: intramural neurons, revealing a colocalization of dynorphin-A(1-17) and dynorphin-A(1-8), were round, contained a large and round nucleus and were lying sporadically in the longitudinal muscle layer as well as bulb-shaped neurons expressing only a dynorphin-A(1-8) immunoreactivity. The latter were recognized abundantly in the myenteric plexus. Myenteric plexus nerve fibres and terminals were immunoreactive for dynorphin-A(1-8), but not for dynorphin-A(1-17). Dynorphin-A(1-8) immunostained nerve terminals formed close contacts with large non-dynorphinergic myenteric plexus perikarya. These findings might indicate that dynorphin-A(1-8) is processed directly from its prodynorphin ('preproenkephalin B') precursor within myenteric plexus perikarya and indirectly via dynorphin-A(1-17) within intramural perikarya, indicating the presence of two separate dynorphinergic systems in the rat duodenum.
用分别针对阿片肽强啡肽 -A(1 - 17)和强啡肽 -A(1 - 8)的特异性抗血清，对大鼠十二指肠的相邻连续切片进行交替免疫荧光显微镜研究染色。这一结果证明，在那里存在两个独立的强啡肽能神经元群体：壁内神经元呈圆形，含有一个大的圆形细胞核，散在于纵肌层，显示强啡肽 -A(1 - 17)和强啡肽 -A(1 - 8)共定位；还有仅表达强啡肽 -A(1 - 8)免疫反应性的灯泡状神经元，后者在肌间神经丛中大量存在。肌间神经丛神经纤维和终末对强啡肽 -A(1 - 8)有免疫反应，但对强啡肽 -A(1 - 17)无免疫反应。强啡肽 -A(1 - 8)免疫染色的神经终末与大型非强啡肽能肌间神经丛核周体形成紧密接触。这些发现可能表明，强啡肽 -A(1 - 8)在肌间神经丛核周体内直接从其前强啡肽（“前脑啡肽原B”）前体加工而来，而在壁内核周体内则通过强啡肽 -A(1 - 17)间接加工，这表明大鼠十二指肠中存在两个独立的强啡肽能系统。
