State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
Bioresour Technol. 2017 Oct;241:802-811. doi: 10.1016/j.biortech.2017.05.192. Epub 2017 Jun 1.
Low biomass output is a crucial reason for low polyhydroxyalkanoate (PHA) production in mixed microbial cultures (MMCs) PHA process. In this research, an extended cultivation strategy was proposed to rapidly expand the biomass yield of PHA accumulating MMCs and conserve the PHA accumulating ability simultaneously. High PHA content of the cultivated MMCs of 71.4% and 66.7% (higher than 62.1% of the seed biomass) in batch assays and biomass magnification of 43 and 52 were obtained after 10days of extended cultivation with and without sludge discharge, respectively. By embedding the extended cultivation process into the production process, a highly competitive PHA production performance in terms of overall PHA storage yield (0.49g CODPHA/gCODVFA) and volumetric productivity (1.21gPHA/L/d with final cell density of 17.22g/L) was achieved. The proposed PHA production process based on the extended cultivation can be a promising choice in industrial scale practice.
低生物质产量是混合微生物培养（MMC）PHA 工艺中聚羟基烷酸酯（PHA）产量低的一个关键原因。在这项研究中，提出了一种扩展培养策略，以快速提高PHA 积累 MMC 的生物量产量，并同时保持 PHA 积累能力。在批式实验中，培养的 MMC 的 PHA 含量分别高达 71.4%和 66.7%（高于种子生物量的 62.1%），经过 10 天的扩展培养，分别有和没有污泥排放时，生物量放大倍数为 43 和 52。通过将扩展培养过程嵌入到生产过程中，在总体 PHA 储存产率（0.49g CODPHA/gCODVFA）和容积产率（1.21gPHA/L/d，最终细胞密度为 17.22g/L）方面实现了极具竞争力的 PHA 生产性能。基于扩展培养的提出的 PHA 生产工艺在工业规模实践中可能是一个有前景的选择。
