Deng Jinchuan, Yuan Shuheng, Li Xin, Wang Kebing, Xie Lingxia, Li Na, Wang Jin, Huang Nan
Key Lab. of Advanced Technology for Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China; The Institute of Biomaterials and Surface Engineering, Southwest Jiaotong University, Chengdu 610031, China.
Key Lab. of Advanced Technology for Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China; The Institute of Biomaterials and Surface Engineering, Southwest Jiaotong University, Chengdu 610031, China.
Mater Sci Eng C Mater Biol Appl. 2017 Oct 1;79:305-314. doi: 10.1016/j.msec.2017.05.057. Epub 2017 May 10.
Good hemocompatibility and rapid endothelialization are two key factors in the success of stent interventional therapy. In this study, aptamers with the ability to capture endothelial progenitors and anticoagulant molecular heparin were successfully immobilized on the surface of dopamine/polyethylenimine (PDA/PEI) copolymer coating via electrostatic interaction. The results of X-ray spectroscopy (XPS), water contact angle (WCA), and immunofluorescence staining tests confirmed the successful introduction of heparin and aptamers. Platelet adhesion and whole blood experiments demonstrated that the hemocompatibility of the co-modified surface was improved. Dynamic endothelial progenitor cell (EPC) capture experiments showed that the modified surfaces could effectively capture the endothelial progenitor in dynamic conditions. More importantly, ex vivo experiments revealed that the modified surfaces could regulate the distribution of CD34/vWF-positive cells on stent surfaces, and this was beneficial for the endothelialization of vascular stents. These results suggested that heparin and aptamer co-modified stents could capture EPCs and promote endothelialization. This surface co-modification strategy has great potential for enhancing stent development.
良好的血液相容性和快速内皮化是支架介入治疗成功的两个关键因素。在本研究中，具有捕获内皮祖细胞能力的适配体和抗凝分子肝素通过静电相互作用成功固定在多巴胺/聚乙烯亚胺（PDA/PEI）共聚物涂层表面。X射线光谱（XPS）、水接触角（WCA）和免疫荧光染色测试结果证实了肝素和适配体的成功引入。血小板黏附实验和全血实验表明，共修饰表面的血液相容性得到了改善。动态内皮祖细胞（EPC）捕获实验表明，修饰后的表面能够在动态条件下有效捕获内皮祖细胞。更重要的是，体外实验表明，修饰后的表面能够调节支架表面CD34/vWF阳性细胞的分布，这有利于血管支架的内皮化。这些结果表明，肝素和适配体共修饰支架能够捕获EPC并促进内皮化。这种表面共修饰策略在增强支架研发方面具有巨大潜力。
