CD36参与调节急性棕榈酸刺激诱导的N1E-115细胞中NPY和AgRP的减少。
Involvement of CD36 in Modulating the Decrease of NPY and AgRP Induced by Acute Palmitic Acid Stimulation in N1E-115 Cells.
作者信息
Ma Yan, Wang Xiaoyi, Yang Hongying, Zhang Xu, Yang Nianhong
机构信息
Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Ministry of Education Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
出版信息
Nutrients. 2017 Jun 17;9(6):626. doi: 10.3390/nu9060626.
Central nervous system (CNS) fatty acid sensing plays an important role in the regulation of food intake, and palmitic acid (PA) is the most important long chain fatty acid (LCFA) in the mammalian diet. To explore the effect of PA on central neuropeptide expression and the role of the cluster of the differentiation of 36 (CD36) in the process, N1E-115 cells were cultured with PA in the presence or absence of sulfosuccinimidyl-oleate (SSO), a CD36 inhibitor. Results showed that 10 μmol/L PA significantly reduced NPY and AgRP mRNA expression after 20 min of exposure, while the expression of CD36 was upregulated. The presence of SSO significantly attenuated the decrease of NPY and AgRP expression that was induced by PA alone, although no notable effect on PA- induced CD36 gene expression was observed. In conclusion, our study suggests the involvement of CD36 in the PA-induced decrease of NPY and AgRP in N1E-115 cells.
中枢神经系统（CNS）的脂肪酸感知在食物摄入调节中起重要作用，而棕榈酸（PA）是哺乳动物饮食中最重要的长链脂肪酸（LCFA）。为了探究PA对中枢神经肽表达的影响以及分化簇36（CD36）在此过程中的作用，在存在或不存在CD36抑制剂磺基琥珀酰亚胺油酸酯（SSO）的情况下，用PA培养N1E-115细胞。结果显示，暴露20分钟后，10μmol/L的PA显著降低了NPY和AgRP mRNA的表达，而CD36的表达上调。SSO的存在显著减弱了单独PA诱导的NPY和AgRP表达的降低，尽管未观察到其对PA诱导的CD36基因表达有显著影响。总之，我们的研究表明CD36参与了PA诱导的N1E-115细胞中NPY和AgRP的降低。
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