Buranaamnuay K
Reproductive Biology Research Group, Institute of Molecular Biosciences (MB), Mahidol University, 73170 Nakhon Pathom, Thailand.
Anim Reprod Sci. 2017 Aug;183:56-65. doi: 10.1016/j.anireprosci.2017.06.002. Epub 2017 Jun 13.
The main objectives of sperm cryopreservation in domestic cats are to preserve these gametes for future use, especially in valuable domestic cat breeds and to use knowledge-gained for developing sperm preservation techniques in wild felids that are threatened with extinction. To achieve acceptable quality of post-thaw sperm and results after insemination, sperm samples must be properly handled, cryopreserved and thawed by using appropriate protocols. In this paper, cryopreservation protocols of domestic cat sperm that have been reported previously are described. The subtopics include sources of sperm, freezing extenders, methods of sperm dilution, freezing storage vessels, methods of sperm cryopreservation, thawing temperature, and thawing extenders. In addition, comparisons of sperm quality results for different treatments within the same studies and between different studies are also presented.
家猫精子冷冻保存的主要目的是保存这些配子以供未来使用，特别是在珍贵的家猫品种中，并利用所获得的知识来开发濒危野生猫科动物的精子保存技术。为了获得可接受的解冻后精子质量和授精后的结果，必须使用适当的方案对精子样本进行妥善处理、冷冻保存和解冻。本文描述了先前报道的家猫精子冷冻保存方案。子主题包括精子来源、冷冻稀释液、精子稀释方法、冷冻储存容器、精子冷冻保存方法、解冻温度和解冻稀释液。此外，还展示了同一研究中不同处理之间以及不同研究之间精子质量结果的比较。
