用表达奇异变形杆菌ompA的重组乳酸乳球菌作为泰山马尾松花粉多糖佐剂进行口服免疫，可使小鼠获得最佳保护。
Oral immunisation with Taishan Pinus massoniana pollen polysaccharide adjuvant with recombinant Lactococcus lactis-expressing Proteus mirabilis ompA confers optimal protection in mice.
作者信息
Zhou J, Wei K, Wang C, Dong W, Ma N, Zhu L, Hu L P, Huang H, Zhu R
机构信息
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, Shandong Taian 271018, PR China.
Animal Disease Prevention and Control Center of Shandong Province, Animal Husbandry and Veterinary Bureau of Shandong Province, Shandong Jinan 250022, PR China.
出版信息
Allergol Immunopathol (Madr). 2017 Sep-Oct;45(5):496-505. doi: 10.1016/j.aller.2017.04.005. Epub 2017 Jun 16.
BACKGROUND
Proteus mirabilis poses a critical burden on the breeding industry, but no efficient vaccine is available for animals.
METHOD
A recombinant Lactococcus lactis expressing the ompA of P. mirabilis was used to develop a vaccine. The mucosal and systemic immune responses of the recombinant vaccine were evaluated in mice after oral immunisation. The inhibition on P. mirabilis colonisation of vaccines was also determined. Moreover, Taishan Pinus massoniana pollen polysaccharides (TPPPS) were used as adjuvants to examine the immunomodulatory effects.
RESULTS
The pure recombinant L. lactis vaccine significantly induced the production of specific IgA and IgG, IL-2, IL-4, IFN-γ, and T lymphocyte proliferation, and the immunised mice exhibited significant resistance to P. mirabilis colonisation. Notably, the TPPPS adjuvant vaccines induced higher levels of immune responses than the pure L. lactis.
CONCLUSIONS
The L. lactis as a vaccine vehicle combined with TPPPS adjuvant provides a feasible method for preventing P. mirabilis infection.
背景
奇异变形杆菌给养殖业带来了严重负担，但目前尚无针对动物的有效疫苗。
方法
利用表达奇异变形杆菌ompA的重组乳酸乳球菌研制疫苗。口服免疫后，在小鼠中评估重组疫苗的黏膜和全身免疫反应。还测定了疫苗对奇异变形杆菌定植的抑制作用。此外，使用泰山马尾松花粉多糖（TPPPS）作为佐剂来研究其免疫调节作用。
结果
纯重组乳酸乳球菌疫苗显著诱导了特异性IgA和IgG、IL-2、IL-4、IFN-γ的产生以及T淋巴细胞增殖，且免疫小鼠对奇异变形杆菌定植表现出显著抗性。值得注意的是，TPPPS佐剂疫苗诱导的免疫反应水平高于纯乳酸乳球菌疫苗。
结论
乳酸乳球菌作为疫苗载体并结合TPPPS佐剂为预防奇异变形杆菌感染提供了一种可行的方法。
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