A Key Enzyme of the NAD Salvage Pathway in Thermus thermophilus: Characterization of Nicotinamidase and the Impact of Its Gene Deletion at High Temperatures.

NAD (NAD) is a cofactor related to many cellular processes. This cofactor is known to be unstable, especially at high temperatures, where it chemically decomposes to nicotinamide and ADP-ribose. Bacteria, yeast, and higher organisms possess the salvage pathway for reconstructing NAD from these decomposition products; however, the importance of the salvage pathway for survival is not well elucidated, except for in pathogens lacking the NAD synthesis pathway. Herein, we report the importance of the NAD salvage pathway in the thermophilic bacterium HB8 at high temperatures. We identified the gene encoding nicotinamidase (TTHA0328), which catalyzes the first reaction of the NAD salvage pathway. This recombinant enzyme has a high catalytic activity against nicotinamide ( of 17 μM, of 50 s, / of 3.0 × 10 s · mM). Deletion of this gene abolished nicotinamide deamination activity in crude extracts of and disrupted the NAD salvage pathway in Disruption of the salvage pathway led to the severe growth retardation at a higher temperature (80°C), owing to the drastic decrease in the intracellular concentrations of NAD and NADH. NAD and other nicotinamide cofactors are essential for cell metabolism. These molecules are unstable and decompose, even under the physiological conditions in most organisms. Thermophiles can survive at high temperatures where NAD decomposition is, in general, more rapid. This study emphasizes that NAD instability and its homeostasis can be one of the important factors for thermophile survival in extreme temperatures.