Tazawa H, Lomholt J P, Johansen K
Comp Biochem Physiol A Comp Physiol. 1985;81(3):641-2. doi: 10.1016/0300-9629(85)91040-0.
A technique is described for direct measurement of allantoic blood flow in chicken embryos in situ using an electromagnetic flow meter. A branch of the allantoic artery was catheterized non-obstructively for simultaneous pressure measurement. In 15-day-old embryos at 39 degrees C the mean heart rate was 278 beats/min falling to 124 beats/min at 26 degrees C. Despite this fall in heart rate, blood flow fell only 10% from 4.4 ml/min at 39 degrees C to 4.0 ml/min at 26 degrees C. Mean allantoic blood pressure was similarly nearly unaffected by temperature. Allantoic blood flow was insignificantly altered by hyperoxic as well as hypoxic exposure (6% O2).
本文描述了一种使用电磁流量计直接测量鸡胚原位尿囊血流的技术。对尿囊动脉的一个分支进行无阻塞插管，以便同时测量压力。在39℃的15日龄胚胎中，平均心率为278次/分钟，在26℃时降至124次/分钟。尽管心率下降，但血流仅从39℃时的4.4毫升/分钟下降10%至26℃时的4.0毫升/分钟。平均尿囊血压同样几乎不受温度影响。高氧以及低氧暴露（6%氧气）对尿囊血流的影响不显著。
