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家鸡尿囊血流的直接测量。对环境温度和氧分压变化的反应。

Direct measurement of allantoic blood flow in the chicken, Gallus domesticus. Responses to alteration in ambient temperature and PO2.

作者信息

Tazawa H, Lomholt J P, Johansen K

出版信息

Comp Biochem Physiol A Comp Physiol. 1985;81(3):641-2. doi: 10.1016/0300-9629(85)91040-0.

Abstract

A technique is described for direct measurement of allantoic blood flow in chicken embryos in situ using an electromagnetic flow meter. A branch of the allantoic artery was catheterized non-obstructively for simultaneous pressure measurement. In 15-day-old embryos at 39 degrees C the mean heart rate was 278 beats/min falling to 124 beats/min at 26 degrees C. Despite this fall in heart rate, blood flow fell only 10% from 4.4 ml/min at 39 degrees C to 4.0 ml/min at 26 degrees C. Mean allantoic blood pressure was similarly nearly unaffected by temperature. Allantoic blood flow was insignificantly altered by hyperoxic as well as hypoxic exposure (6% O2).

摘要

本文描述了一种使用电磁流量计直接测量鸡胚原位尿囊血流的技术。对尿囊动脉的一个分支进行无阻塞插管,以便同时测量压力。在39℃的15日龄胚胎中,平均心率为278次/分钟,在26℃时降至124次/分钟。尽管心率下降,但血流仅从39℃时的4.4毫升/分钟下降10%至26℃时的4.0毫升/分钟。平均尿囊血压同样几乎不受温度影响。高氧以及低氧暴露(6%氧气)对尿囊血流的影响不显著。

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