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淡水和海水适应型虹鳟(Salmo gairdnerii R.)肠道中的钠转运及酶活性

Na transport and enzyme activities in the intestine of the freshwater and sea-water adapted trout (Salmo gairdnerii R.).

作者信息

Colin D A, Nonnotte G, Leray C, Nonnotte L

出版信息

Comp Biochem Physiol A Comp Physiol. 1985;81(3):695-8. doi: 10.1016/0300-9629(85)91049-7.

DOI:10.1016/0300-9629(85)91049-7
PMID:2863061
Abstract

Unidirectional fluxes of Na+ obtained in perfused preparation and mucosal enzyme equipment (alkaline phosphatase, ouabain-sensitive Na+, K+-ATPase) have been determined in the middle and posterior intestine of freshwater (FW) and sea-water (SW) adapted trout. In FW, influxes and outfluxes were higher in the middle than in the posterior intestine, although net fluxes were similar. SW adaptation induced an increase of influxes and net fluxes mainly in the posterior intestine. SW adaptation decreased the alkaline phosphatase activity only in the posterior intestine. Na+,K+-ATPase activity was always higher in the middle than in the posterior intestine in FW and SW and increased in both parts by SW adaptation. Thus, it seems that SW adaptation of rainbow trout modifies Na intestinal absorption principally in its posterior part and in relation with the Na+, K+-ATPase activity.

摘要

在淡水(FW)和海水(SW)适应的虹鳟鱼的中肠和后肠中,已测定了在灌注制剂和黏膜酶设备(碱性磷酸酶、哇巴因敏感的Na⁺、K⁺-ATP酶)中获得的Na⁺单向通量。在淡水中,中肠的流入和流出通量高于后肠,尽管净通量相似。海水适应主要在后肠诱导了流入通量和净通量的增加。海水适应仅在后肠降低了碱性磷酸酶活性。在淡水和海水中,中肠的Na⁺、K⁺-ATP酶活性始终高于后肠,并且通过海水适应,两个部位的该酶活性均增加。因此,似乎虹鳟鱼的海水适应主要在其后肠改变了Na⁺的肠道吸收,且与Na⁺、K⁺-ATP酶活性有关。

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