Ma Ningqiang, Shen Weiwei, Pang Hailin, Zhang Ning, Shi Hong, Wang Jianlin, Zhang Helong
1 Department of Oncology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.
2 Department of Hepatobiliary Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
Tumour Biol. 2017 Jun;39(6):1010428317700405. doi: 10.1177/1010428317700405.
Bone is the third most common site of cancer metastasis. In total, 30%-40% of lung cancer cases can develop skeletal metastasis for which no effective therapy in clinic is available. RCAN1 (regulator of calcineurin 1) is an important regulator in angiogenesis which is vital to tumor growth. In this study, we investigated the changes of biological behaviors in SBC-5 and SBC-3 cells after the RCAN1 expression level was changed. Briefly, overexpression of RCAN1 significantly attenuated their malignancy, including decreased ability of proliferation, colony formation, migration, invasion, and bone adherence. Furthermore, the cell cycle progression was impeded. Although the opposite changes were observed in SBC-3 cells after the RCAN1 expression was suppressed by RNA interference, the apoptosis rate was not affected by the expression level of RCAN1 in these cells. So, our research revealed that RCAN1 was involved in the development of small cell lung cancer, and it might be a cancer-inhibiting gene for the formation of bone metastases in small cell lung cancer.
骨是癌症转移的第三大常见部位。总体而言，30%-40%的肺癌病例会发生骨转移，临床上对此尚无有效的治疗方法。RCAN1（钙调神经磷酸酶调节因子1）是血管生成中的一个重要调节因子，对肿瘤生长至关重要。在本研究中，我们研究了RCAN1表达水平改变后SBC-5和SBC-3细胞生物学行为的变化。简而言之，RCAN1的过表达显著减弱了它们的恶性程度，包括增殖能力、集落形成能力、迁移能力、侵袭能力和骨黏附能力下降。此外，细胞周期进程受到阻碍。虽然在通过RNA干扰抑制RCAN1表达后，SBC-3细胞出现了相反的变化，但这些细胞的凋亡率不受RCAN1表达水平的影响。因此，我们的研究表明RCAN1参与了小细胞肺癌的发展，它可能是小细胞肺癌骨转移形成的一个抑癌基因。
