Joh T H, Baetge E E, Ross M E, Lai C Y, Docherty M, Bradford H, Reis D J
Fed Proc. 1985 Sep;44(12):2773-9.
We found that the catecholamine biosynthetic enzymes tyrosine hydroxylase (TH) (EC 1.14.16.2), dopamine beta-hydroxylase (EC 1.14.17.1), and phenylethanolamine N-methyltransferase (EC 2.1.1.28) share similar protein domains in their primary structures and that they share common gene coding sequences. In a recent report we also demonstrated that antiserums directed against choline acetyltransferase (EC 2.3.1.6), glutamic acid decarboxylase (EC 4.1.1.15), and TH cause specific complement-mediated lysis of cholinergic, gamma-aminobutyric acid-ergic, and dopaminergic subpopulations of synaptosomes, respectively. This interaction of specific antibodies to the specific subpopulation of synaptosomal membrane, e.g., recognition of antibody to TH to only the dopaminergic subpopulation of synaptosomal membrane protein, indicates that the neurotransmitter enzyme and membrane protein of its own synaptosomes may also share common protein domains. Therefore, we postulate that the specific neurotransmitter biosynthetic enzyme and a certain membrane protein of the nerve endings may share similar gene coding sequences, and that expression of these proteins may determine the phenotype of the neuron.
我们发现儿茶酚胺生物合成酶酪氨酸羟化酶（TH）（EC 1.14.16.2）、多巴胺β-羟化酶（EC 1.14.17.1）和苯乙醇胺N-甲基转移酶（EC 2.1.1.28）在其一级结构中具有相似的蛋白质结构域，并且它们共享共同的基因编码序列。在最近的一份报告中，我们还证明，针对胆碱乙酰转移酶（EC 2.3.1.6）、谷氨酸脱羧酶（EC 4.1.1.15）和TH的抗血清分别导致突触体胆碱能、γ-氨基丁酸能和多巴胺能亚群的特异性补体介导的裂解。特异性抗体与突触体膜特异性亚群的这种相互作用，例如，抗TH抗体仅识别突触体膜蛋白的多巴胺能亚群，表明神经递质酶及其自身突触体的膜蛋白也可能共享共同的蛋白质结构域。因此，我们推测神经末梢的特异性神经递质生物合成酶和某种膜蛋白可能共享相似的基因编码序列，并且这些蛋白质的表达可能决定神经元的表型。
