Sahul Hameed A S, Abdul Majeed S, Vimal S, Madan N, Rajkumar T, Santhoshkumar S, Sivakumar S
OIE Reference Laboratory for WTD, Department of Zoology, C. Abdul Hakeem College, Melvisharam, Tamil Nadu, India.
J Fish Dis. 2017 Dec;40(12):1823-1830. doi: 10.1111/jfd.12655. Epub 2017 Jun 20.
Whiteleg shrimp, Litopenaeus vannamei, with clinical sign of muscle opaqueness with reddish colour at the distal abdominal segments were observed in farms located in West Bengal State, India. The mortality of shrimp in all disease outbreak ponds ranged from 20% to 50%, and mortality increased gradually. The RT-PCR assay of these samples using primer sets specific to infectious myonecrosis virus (IMNV) revealed its presence in the disease outbreak ponds. The IMNV infection was reproduced in healthy shrimp by intramuscular injection to satisfy River's postulates. The virus caused mortality in intramuscularly challenged shrimp, but failed to cause mortality by oral route. Tissue distribution of IMNV in infected shrimp by RT-PCR assay revealed the presence of this virus in haemolymph, gill, hepatopancreas and muscle. This study confirms that the disease outbreak which occurred in the shrimp farms located at Purba Medinipur District, West Bengal, India, was due to IMNV.
在印度西孟加拉邦的养殖场中，发现凡纳滨对虾（Litopenaeus vannamei）出现了腹部末端肌肉浑浊且呈红色的临床症状。在所有发病池塘中，对虾的死亡率在20%至50%之间，且死亡率逐渐上升。使用针对传染性肌坏死病毒（IMNV）的引物对这些样本进行RT-PCR检测，结果显示在发病池塘中存在该病毒。通过肌肉注射将IMNV感染接种到健康对虾体内，以满足里弗斯法则。该病毒在肌肉注射感染的对虾中导致了死亡，但通过口服途径未能导致死亡。通过RT-PCR检测IMNV在感染对虾中的组织分布，结果显示在血淋巴、鳃、肝胰腺和肌肉中均存在该病毒。本研究证实，印度西孟加拉邦普尔巴梅迪尼布尔区养殖场发生的疾病暴发是由IMNV引起的。
