Perrott P, Turgeon N, Gauthier-Levesque L, Duchaine C
Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec, Université Laval, Québec City, QC, Canada.
Département de biochimie, de microbiologie et de bio-informatique, Faculté des sciences et de génie, Université Laval, Québec City, QC, Canada.
J Appl Microbiol. 2017 Sep;123(3):688-697. doi: 10.1111/jam.13514. Epub 2017 Aug 3.
Little is known about how bacteria are aerosolized in terms of whether some bacteria will be found in the air more readily than others that are present in the source. This report describes in vitro experiments to compare aerosolization rates (also known as preferential aerosolization) of Gram-positive and Gram-negative bacteria as well as rod- and coccus-shaped bacteria, using two nebulization conditions.
A consortium of five bacterial species was aerosolized in a homemade chamber. Aerosols generated with a commercial nebulizer and a homemade bubble-burst aerosol generator were compared. Data suggest that Pseudomonas aeruginosa was preferentially aerosolized in comparison to Moraxella catarrhalis, Lactobacillus paracasei, Staphylococcus aureus and Streptococcus suis, independently of the method of aerosolization. Bacterial integrity of Strep. suis was more preserved compared to other bacteria studied as revealed with PMA-qPCR.
We reported the design of an aerosol chamber and bubble-burst generator for the in vitro study of preferential aerosolization. In our setting, preferential aerosolization was influenced by bacterial properties instead of aerosolization mechanism.
These findings could have important implications for predicting the composition of bioaerosols in various locations such as wastewater treatment plants, agricultural settings and health care settings.
关于细菌如何形成气溶胶，即与源中存在的其他细菌相比，某些细菌是否更容易在空气中被发现，目前所知甚少。本报告描述了体外实验，使用两种雾化条件比较革兰氏阳性菌和革兰氏阴性菌以及杆状菌和球菌的雾化率（也称为优先雾化）。
在一个自制的腔室内将五种细菌的混合菌群雾化。比较了使用商用雾化器和自制气泡破裂雾化发生器产生的气溶胶。数据表明，与卡他莫拉菌、副干酪乳杆菌、金黄色葡萄球菌和猪链球菌相比，铜绿假单胞菌优先雾化，且与雾化方法无关。如通过PMA-qPCR所揭示的，与其他所研究的细菌相比，猪链球菌的细菌完整性得到了更好的保留。
我们报告了一种用于优先雾化体外研究的气溶胶腔室和气泡破裂发生器的设计。在我们的实验环境中，优先雾化受细菌特性而非雾化机制的影响。
这些发现对于预测废水处理厂、农业环境和医疗环境等不同场所生物气溶胶的组成可能具有重要意义。
