Ravdin J I, Murphy C F, Guerrant R L, Long-Krug S A
J Infect Dis. 1985 Sep;152(3):542-9. doi: 10.1093/infdis/152.3.542.
The in vitro mechanisms by which Entamoeba histolytica trophozoites lyse target Chinese hamster ovary (CHO) cells were examined. Calcium chelators ethylenediaminetetraacetate and ethyleneglycol bis (beta-aminoethyl ether)-N,N'-tetraacetate (10 mM) inhibited amebic cytolysis of target CHO cells (P less than .01). A putative antagonist of intracellular calcium flux, 8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate (TMB-8; greater than or equal to 250 microM), inhibited amebic adherence and cytolysis (P less than .001). Quinacrine, Rosenthal's inhibitor (dimethyl-dl-2,3-distearoyloxypropyl-2'-hydroxyethyl ammonium acetate), phosphatidylcholine, and hydrocortisone (greater than or equal to 10(-4) M), all pharmacological antagonists of eukaryotic phospholipase A enzymes, inhibited amebic killing of target CHO cells (P less than .001). At 37 C quinacrine and hydrocortisone reduced amebic adherence to CHO cells, whereas Rosenthal's inhibitor and phosphatidylcholine did not. Phosphatidylcholine and TMB-8 demonstrated a synergistic inhibitory effect on amebic killing of target CHO cells (P less than .001). These studies indicate that extracellular calcium ions, amebic intracellular calcium flux, and amebic phospholipase A activity are required for cytolysis of target cells by E. histolytica.
我们研究了溶组织内阿米巴滋养体裂解靶细胞中国仓鼠卵巢（CHO）细胞的体外机制。钙螯合剂乙二胺四乙酸和乙二醇双（β-氨基乙基醚）-N，N'-四乙酸（10 mM）抑制了靶CHO细胞的阿米巴细胞溶解作用（P <.01）。一种假定的细胞内钙通量拮抗剂8-（N，N-二乙氨基）辛基-3,4,5-三甲氧基苯甲酸酯（TMB-8；≥250 μM）抑制了阿米巴的黏附和细胞溶解作用（P <.001）。喹吖因、罗森塔尔抑制剂（二甲基-dl-2,3-二硬脂酰氧基丙基-2'-羟乙基乙酸铵）、磷脂酰胆碱和氢化可的松（≥10^-4 M），所有这些真核磷脂酶A酶的药理学拮抗剂，都抑制了靶CHO细胞的阿米巴杀伤作用（P <.001）。在37℃时，喹吖因和氢化可的松减少了阿米巴对CHO细胞的黏附，而罗森塔尔抑制剂和磷脂酰胆碱则没有。磷脂酰胆碱和TMB-8对靶CHO细胞的阿米巴杀伤作用表现出协同抑制作用（P <.001）。这些研究表明，细胞外钙离子、阿米巴细胞内钙通量和阿米巴磷脂酶A活性是溶组织内阿米巴裂解靶细胞所必需的。
