Kishen R, Pleuvry B J
J Pharm Pharmacol. 1985 Jul;37(7):502-4. doi: 10.1111/j.2042-7158.1985.tb03052.x.
The smooth muscle relaxant actions of sodium nitroprusside and glyceryl trinitrate have been compared to those of aminophylline and isoprenaline on isolated guinea pig trachealis muscle. Ethacrynic acid (0.25 X 10(-4) M), an alkylator of sulphydryl groups, interacted differently with the four agents. In the presence of ethacrynic acid the concentration response curve of the muscle preparation to sodium nitroprusside and glyceryl trinitrate was shifted to the higher concentration ranges and the maximum response was severely reduced. The concentration response curve for isoprenaline was shifted to the higher concentration ranges with no change in the maximum response and the response to aminophylline was unchanged. These results argue against common intermediate sites of action involving sulphydryl groups of the four agents in guinea-pig trachealis.
已将硝普钠和硝酸甘油的平滑肌松弛作用与氨茶碱和异丙肾上腺素对豚鼠离体气管平滑肌的作用进行了比较。乙磺半胱氨酸（0.25×10⁻⁴M），一种巯基烷化剂，与这四种药物的相互作用方式不同。在乙磺半胱氨酸存在下，肌肉制剂对硝普钠和硝酸甘油的浓度反应曲线向更高浓度范围移动，且最大反应严重降低。异丙肾上腺素的浓度反应曲线向更高浓度范围移动，最大反应无变化，而对氨茶碱的反应不变。这些结果表明，豚鼠气管中这四种药物不存在涉及巯基的共同中间作用位点。
