Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon 34129, Republic of Korea.
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea; Advanced Biomass R&D Center, Daejeon 34141, Republic of Korea.
Bioresour Technol. 2017 Nov;244(Pt 2):1317-1328. doi: 10.1016/j.biortech.2017.06.038. Epub 2017 Jun 12.
The microalgae-based biorefinement process has attracted much attention from academic and industrial researchers attracted to its biofuel, food and nutraceutical applications. In this paper, recent developments in cell-disruption and lipid-extraction methods, focusing on four biotechnologically important microalgal species (namely, Chlamydomonas, Haematococcus, Chlorella, and Nannochloropsis spp.), are reviewed. The structural diversity and rigidity of microalgal cell walls complicate the development of efficient downstream processing methods for cell-disruption and subsequent recovery of intracellular lipid and pigment components. Various mechanical, chemical and biological cell-disruption methods are discussed in detail and compared based on microalgal species and status (wet/dried), scale, energy consumption, efficiency, solvent extraction, and synergistic combinations. The challenges and prospects of the downstream processes for the future development of eco-friendly and economical microalgal biorefineries also are outlined herein.
基于微藻的生物炼制过程引起了学术界和工业界研究人员的极大关注，因为它在生物燃料、食品和营养保健品方面具有应用潜力。本文综述了近年来在细胞破碎和脂质提取方法方面的最新进展，重点介绍了四种具有生物技术重要性的微藻物种（即，衣藻、血球藻、小球藻和拟南芥属）。微藻细胞壁的结构多样性和刚性使开发高效的下游细胞破碎方法以及随后回收细胞内脂质和色素成分变得复杂。详细讨论了各种机械、化学和生物学细胞破碎方法，并根据微藻种类和状态（湿/干）、规模、能耗、效率、溶剂提取和协同组合进行了比较。本文还概述了下游工艺的挑战和前景，以期为环保和经济的微藻生物炼制厂的未来发展提供参考。
