Singapore Bioimaging Consortium, Agency for Science, Technology and Research, 11 Biopolis Way, No. 02-02 Helios, Singapore 138667.
Department of Pharmacy, Sunchon National University, 255 Jungangno, Suncheon 540-950, Republic of Korea.
J Immunol Res. 2017;2017:2471627. doi: 10.1155/2017/2471627. Epub 2017 May 29.
Lectins are carbohydrate-binding proteins with various biological activities, such as antitumor and immunomodulatory effects. Although lectins have various biological activities, they are still limited by cytotoxicity in normal cells. To overcome this problem, we used the noncytotoxic part of Korean mistletoe lectin B-chain (KML-B) to induce maturation of dendritic cells (DCs). A previous study reported that KML-B induces DC maturation by triggering TLR-4, including expression of costimulatory molecules (CD40, CD80, and CD86), MHC II, and secretion of cytokines in DCs. Additionally, matured DCs by KML-B induced T helper (Th) cell activation and differentiation toward Th1 cells. However, the interaction of KML-B-treated DCs with CD8 T cells is still poorly understood. In this study, we confirmed the ability of matured DCs by KML-B to stimulate cytotoxic T cells using OT-1 mouse-derived CD8 T cells. KML-B induced MHC I expression in DCs, stimulation of CD8 T cell activation and proliferation, and IFN- secretion. Moreover, tumor sizes were reduced by KML-B treatment during vaccination of OVA-pulsed DCs. Here, we confirmed induction of CD8 T cell activation and the antitumor effect of KML-B treatment in DCs.
凝集素是具有多种生物活性的碳水化合物结合蛋白，如抗肿瘤和免疫调节作用。尽管凝集素有多种生物活性，但它们在正常细胞中仍受到细胞毒性的限制。为了克服这个问题，我们使用韩国槲寄生凝集素 B 链（KML-B）的非细胞毒性部分诱导树突状细胞（DC）成熟。先前的研究表明，KML-B 通过触发 TLR-4 诱导 DC 成熟，包括共刺激分子（CD40、CD80 和 CD86）、MHC II 和 DC 中细胞因子的分泌。此外，KML-B 诱导的成熟 DC 可诱导 T 辅助（Th）细胞向 Th1 细胞激活和分化。然而，KML-B 处理的 DC 与 CD8 T 细胞的相互作用仍知之甚少。在这项研究中，我们使用 OT-1 小鼠来源的 CD8 T 细胞证实了 KML-B 成熟 DC 刺激细胞毒性 T 细胞的能力。KML-B 在 DC 中诱导 MHC I 表达，刺激 CD8 T 细胞活化和增殖，并分泌 IFN-。此外，在 OVA 脉冲 DC 接种时，KML-B 治疗可减小肿瘤大小。在这里，我们证实了 KML-B 在 DC 中诱导 CD8 T 细胞活化和抗肿瘤作用。
