Bray Laura J, Binner Marcus, Freudenberg Uwe, Werner Carsten
Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Dresden, Saxony, Germany.
Science and Engineering Faculty and Institute of Health and Biomedical Innovation, Queensland University of Technology, 60 Musk Avenue, Kelvin Grove, QLD, 4059, Australia.
Methods Mol Biol. 2017;1612:39-63. doi: 10.1007/978-1-4939-7021-6_4.
In situ forming hydrogels prepared from multi-armed poly(ethylene glycol) (PEG), glycosaminoglycans (GAG) and various peptides enable the development of advanced three dimensional (3D) culture models. Herein, we report methods for the PEG-GAG gel-based 3D co-cultivation of human umbilical vein endothelial cells, mesenchymal stromal cells, and different cancer cell lines. The resulting constructs allow for the exploration of interactions between solid tumors with 3D vascular networks in vitro to study the mechanistic aspects of cancer development and to perform drug testing.
由多臂聚乙二醇（PEG）、糖胺聚糖（GAG）和各种肽制备的原位形成水凝胶能够开发先进的三维（3D）培养模型。在此，我们报告了基于PEG-GAG凝胶的人脐静脉内皮细胞、间充质基质细胞和不同癌细胞系的3D共培养方法。所得构建体允许在体外探索实体瘤与3D血管网络之间的相互作用，以研究癌症发展的机制方面并进行药物测试。
