Shi Guofang, Zhan Pengfei, Jin Weiming, Fei JianMing, Zhao Lihua
Huzhou Academy of Agricultural Sciences, Huzhou, Zhejiang, P.R. China.
Arch Insect Biochem Physiol. 2017 Aug;95(4). doi: 10.1002/arch.21397. Epub 2017 Jun 20.
Silkworm (Bombyx mori) is an economically beneficial insect. Its growth and development are regulated by endogenous hormones. In the present study, we found that feeding titanium dioxide nanoparticles (TiO NP) caused a significant increase of body size. TiO NP stimulated the transcription of several genes, including the insulin-related hormone bombyxin, PI3K/Akt/TOR (where PI3K is phosphatidylinositol 3-kinase and TOR is target of rapamycin), and the adenosine 5'-monophosphateactivated protein kinase (AMPK)/target of rapamycin (TOR) pathways. Differentially expressed gene (DEG) analysis documented 26 developmental hormone signaling related genes that were differentially expressed following dietary TiO NP treatment. qPCR analysis confirmed the upregulation of insulin/ecdysteroid signaling genes, such as bombyxin B-1, bombyxin B-4, bombyxin B-7, MAPK, P70S6K, PI3k, eIF4E, E75, ecdysteroid receptor (EcR), and insulin-related peptide binding protein precursor 2 (IBP2). We infer from the upregulated expression of bombyxins and the signaling network that they act in bombyxin-stimulated ecdysteroidogenesis.
家蚕（Bombyx mori）是一种具有经济价值的昆虫。其生长发育受内源激素调控。在本研究中，我们发现喂食二氧化钛纳米颗粒（TiO NP）会导致体型显著增大。TiO NP刺激了多个基因的转录，包括胰岛素相关激素家蚕素、PI3K/Akt/TOR（其中PI3K是磷脂酰肌醇3激酶，TOR是雷帕霉素靶蛋白）以及腺苷5'-单磷酸激活蛋白激酶（AMPK）/雷帕霉素靶蛋白（TOR）信号通路。差异表达基因（DEG）分析记录了26个与发育激素信号相关的基因，这些基因在经饮食TiO NP处理后出现差异表达。qPCR分析证实了胰岛素/蜕皮激素信号基因的上调，如家蚕素B-1、家蚕素B-4、家蚕素B-7、丝裂原活化蛋白激酶（MAPK）、P70S6K、PI3k、真核翻译起始因子4E（eIF4E）、E75、蜕皮激素受体（EcR）以及胰岛素相关肽结合蛋白前体2（IBP2）。我们从家蚕素表达上调以及信号网络推断，它们在家蚕素刺激的蜕皮激素生成过程中发挥作用。
