Amudong Aierken, Muheremu Aikeremujiang, Abudourexiti Tuerhongjiang
Department of Spine Surgery, Sixth Affiliated Hospital of Xinjiang Medical University, Xinjiang Urumqi, China.
J Int Med Res. 2017 Dec;45(6):2036-2041. doi: 10.1177/0300060517711308. Epub 2017 Jun 21.
Objective To explore the relationship between cellular apoptosis and hypertrophy of the ligamentum flavum in the lumbar region. Methods Thirty patients with lumbar spinal stenosis were evaluated. Hypertrophy of the ligamentum flavum was present in 15 patients and absent in 15. Hematoxylin-eosin staining and transforming growth factor beta (TGF-β) immunohistochemical testing were applied to compare these two groups. Results Derangement of fibrous alignment, fibrocartilage changes, and infiltration of inflammatory cells were observed in the patients with hypertrophy of the ligamentum flavum, while fibrous alignment was normal and few inflammatory cells were observed in patients without hypertrophy. Immunohistochemical studies showed positive expression of TGF-β in patients with hypertrophy, while expression was negative in patients without hypertrophy. The integrated optical density was 2.6556708 in the hypertrophy group and 23104671 in the normal controls. Conclusions Expression of TGF-β was closely related to hypertrophy of the ligamentum flavum. Appropriate application of the TGF-β expression level can be used to predict progression of hypertrophy of the ligamentum flavum.
目的 探讨腰椎黄韧带细胞凋亡与肥厚之间的关系。方法 对30例腰椎管狭窄症患者进行评估。其中15例存在黄韧带肥厚，15例无黄韧带肥厚。应用苏木精-伊红染色和转化生长因子β（TGF-β）免疫组织化学检测对两组进行比较。结果 黄韧带肥厚患者可见纤维排列紊乱、纤维软骨改变及炎性细胞浸润，而无肥厚患者纤维排列正常且炎性细胞少见。免疫组织化学研究显示，肥厚患者TGF-β呈阳性表达，无肥厚患者表达为阴性。肥厚组的积分光密度为2.6556708，正常对照组为23104671。结论 TGF-β的表达与黄韧带肥厚密切相关。适当应用TGF-β表达水平可用于预测黄韧带肥厚的进展。
