Saoudi Mongi, Ncir Marwa, Ben Ali Manel, Grati Malek, Jamoussi Kamel, Allouche Noureddine, El Feki Abdelfattah
Laboratory of Animal Ecophysiology, Sciences Faculty, University of Sfax, Sfax, Tunisia.
Gen Physiol Biophys. 2017 Jul;36(3):331-342. doi: 10.4149/gpb_2016057.
In the present study, we evaluated the antioxidant potential of Artemisia campestris essential oil (ACEO) and the possible protective effects against deltamethrin induced hepatic toxic effects. The ACEO showed radical scavenging activity with IC50 = 47.66 ± 2.51 µg/ml, ferric reducing antioxidant power (FRAP) potential (EC50 = 5.36 ± 0.77 µg/ml), superoxide scavenging activity (IC50 = 0.175 ± 0.007 µg/ml) and ˙OH scavenging activity (IC50 = 0.034 ± 0.007 µg/ml). The obtained results of phenolic profile demonstrated that phenolic compounds are the major contributor to the antioxidant activity of ACEO. GC-MS analysis revealed the presence of 61 components in which monoterpene hydrocarbons constitute the major fraction (38.85%). In in vivo study, deltamethrin exposure caused an increase of serum AST, ALT and ALP activities, hepatic malondialdehyde (MDA) (measured as TBARS) and conjugated dienes markers of lipid peroxidation (LPO), while antioxidant enzyme activities (SOD, CAT and GPx) decreased significantly. Furthermore, it induces DNA damage as indicated by DNA fragmentation accompanied with severe histological changes in the liver tissues. The treatment with vitamin E or ACEO significantly improved the hepatic toxicity induced by deltamethrin. It can be concluded that vitamin E and ACEO are able to improve the hepatic oxidative damage induced by deltamethrin. Therefore, ACEO is an important product in reducing the toxic effects of deltamethrin.
在本研究中，我们评估了野艾蒿精油（ACEO）的抗氧化潜力以及对溴氰菊酯诱导的肝脏毒性作用的可能保护作用。ACEO表现出自由基清除活性，IC50 = 47.66 ± 2.51 µg/ml，铁还原抗氧化能力（FRAP）潜力（EC50 = 5.36 ± 0.77 µg/ml），超氧化物清除活性（IC50 = 0.175 ± 0.007 µg/ml）和˙OH清除活性（IC50 = 0.034 ± 0.007 µg/ml）。酚类成分的所得结果表明，酚类化合物是ACEO抗氧化活性的主要贡献者。气相色谱 - 质谱分析显示存在61种成分，其中单萜烃构成主要部分（38.85%）。在体内研究中，溴氰菊酯暴露导致血清AST、ALT和ALP活性增加，肝脏丙二醛（MDA）（以TBARS衡量）和脂质过氧化（LPO）的共轭二烯标志物增加，而抗氧化酶活性（SOD、CAT和GPx）显著降低。此外，如DNA片段化所示，它诱导DNA损伤，并伴有肝脏组织严重的组织学变化。用维生素E或ACEO治疗显著改善了溴氰菊酯诱导的肝脏毒性。可以得出结论，维生素E和ACEO能够改善溴氰菊酯诱导的肝脏氧化损伤。因此，ACEO是降低溴氰菊酯毒性作用的重要产品。
