盐酸氨溴索壳聚糖对大鼠模型术后腹腔粘连形成的预防作用。
The preventive effect of ambroxol hydrochloride chitosan on postoperative intraperitoneal adhesion formation in a rat model.
作者信息
Zhao Jin-Ming, Jin Zhi-Zhong, Zhao Qi-Zhong
机构信息
a Department of 98 Grads , China Medical University , Shenyang , China.
b Department of Emergency , Affiliated First Hospital, China Medical University , Shenyang , China.
出版信息
Acta Chir Belg. 2017 Aug;117(4):232-237. doi: 10.1080/00015458.2017.1287395. Epub 2017 Feb 13.
BACKGROUND
This study aimed to investigate the effects of ambroxol hydrochloride chitosan reduced the degree of peritoneal adhesion.
METHODS
A total of 120 Sprague-Dawley (SD) rats were experimented, group A: the damaged peritoneal was covered with ambroxol hydrochloride chitosan, group B: chitosan membrane only, group C: nothing. Enzyme-linked immunosorbent assay (ELISA), western blotting, and immunohistochemistry (IHC) were used to observe the expression of TNF-α and NF-κB p65.
RESULTS
TNF-α and NF-κB p65 in group A significantly decreased in comparison with the controls. IHC showed TNF-α and NF-κB p65 were significantly down-regulated in group A. Adhesion grade in the treatment group was significantly lower than in controls.
CONCLUSION
Ambroxol hydrochloride chitosan reduced the degree of peritoneal adhesion.
背景
本研究旨在探讨盐酸氨溴索壳聚糖降低腹膜粘连程度的作用。
方法
共对120只Sprague-Dawley（SD）大鼠进行实验，A组：受损腹膜覆盖盐酸氨溴索壳聚糖；B组：仅覆盖壳聚糖膜；C组：不做处理。采用酶联免疫吸附测定（ELISA）、蛋白质印迹法和免疫组织化学（IHC）观察肿瘤坏死因子-α（TNF-α）和核因子-κB p65（NF-κB p65）的表达。
结果
与对照组相比，A组的TNF-α和NF-κB p65显著降低。免疫组织化学显示A组的TNF-α和NF-κB p65显著下调。治疗组的粘连分级显著低于对照组。
结论
盐酸氨溴索壳聚糖降低了腹膜粘连程度。
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