抗氧化剂作为His6-OPH的稳定剂：这对酶来说是它们的一种特殊作用还是常规作用？

Antioxidants as stabilizers for His6-OPH: is this an unusual or regular role for them with enzymes?

作者信息

Efremenko Elena N, Lyagin Ilya V, Cuong Le H, Huong Le M

机构信息

Chemistry Department, Lomonosov Moscow State University, Lenin Hills, 1/3, Moscow 119991, Russia.

Institute of Natural Products Chemistry, VAST, 18 Hoang Quoc Viet, Hanoi, Viet Nam.

出版信息

J Biochem. 2017 Nov 1;162(5):327-334. doi: 10.1093/jb/mvx037.

DOI:10.1093/jb/mvx037

PMID:28637331

Abstract

The effect of 14 different antioxidants on the activity of a hexahistidine-tagged organophosphorus hydrolase (His6-OPH) has been studied in vitro. It has been found that antioxidants can have a positive, neutral or negative effect on the activity of His6-OPH in a native form or in the form of an enzyme-polyelectrolyte complex, while the enzyme itself does not affect their antioxidant activity. A significant stabilizing effect of a number of antioxidants on His6-OPH has been shown against its inhibiting with organic solvents (DMSO and isopropyl alcohol). The kinetics of the process has been studied. Based on molecular docking of all tested antioxidants to the surface of His6-OPH dimer, options of their localization have been identified. These data were used to explain the revealed stabilizing effect of the antioxidants on the enzyme as well as their negative influence on His6-OPH activity.

摘要

已在体外研究了14种不同抗氧化剂对六聚组氨酸标记的有机磷水解酶（His6-OPH）活性的影响。研究发现，抗氧化剂对天然形式或酶-聚电解质复合物形式的His6-OPH活性可产生积极、中性或消极影响，而该酶本身并不影响它们的抗氧化活性。已表明多种抗氧化剂对His6-OPH具有显著的稳定作用，可防止其被有机溶剂（二甲基亚砜和异丙醇）抑制。已研究了该过程的动力学。基于所有测试抗氧化剂与His6-OPH二聚体表面的分子对接，确定了它们的定位方式。这些数据用于解释所揭示的抗氧化剂对该酶的稳定作用及其对His6-OPH活性的负面影响。