Saeedfar Kayvan, Behmanesh Mehrdad, Mortaz Esmaeil, Masjedi Mohammad Reza
Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Chronic Respiratory Diseases Research Center (CRDRC), National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Tanaffos. 2017;16(1):1-8.
Despite being a high burden disorder, the pathogenesis of severe refractory asthma (SRA) is poorly understood. There are some evidences for the involvement of members of the signal transducer and activator of transcription (STAT) family, including and . Our study aimed to evaluate the gene expression of and in asthma and SRA to establish if there is an association.
Using quantitative real-time polymerase chain reactions (qRT-PCR), the transcript levels of and were evaluated in peripheral blood mononuclear lymphocytes (PBML) isolated from 13 patients with SRA, 14 with mild asthma, and 30 healthy volunteers.
There were no significant differences in transcript levels between study groups. There was however a significant difference in transcript levels between cases and controls (p-value=0.03). In comparison to healthy controls, the levels of were notably lower in patients with mild asthma and significantly least in those with SRA.
Our study found no appreciable association between gene expression and either mild asthma or SRA. However, the down regulation in asthmatics and especially SRA is a notable finding which denotes on association between and different level of asthma.
尽管重度难治性哮喘（SRA）是一种负担沉重的疾病，但其发病机制仍知之甚少。有证据表明信号转导和转录激活因子（STAT）家族成员参与其中，包括 和 。我们的研究旨在评估 和 在哮喘和SRA中的基因表达，以确定是否存在关联。
使用定量实时聚合酶链反应（qRT-PCR），评估从13例SRA患者、14例轻度哮喘患者和30名健康志愿者中分离出的外周血单个核细胞（PBML）中 和 的转录水平。
研究组之间 的转录水平无显著差异。然而，病例组和对照组之间 的转录水平存在显著差异（p值 = 0.03）。与健康对照组相比，轻度哮喘患者中 的水平明显较低，而SRA患者中 的水平最低。
我们的研究发现 基因表达与轻度哮喘或SRA之间没有明显关联。然而，哮喘患者尤其是SRA患者中 的下调是一个值得注意的发现，这表明 与不同程度的哮喘之间存在关联。
