Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Munich, Germany.
Department of Pathology, Klinikum Bayreuth, Bayreuth, Germany.
J Immunol Res. 2017;2017:8394593. doi: 10.1155/2017/8394593. Epub 2017 May 30.
infection shows a worldwide prevalence of around 50%. However, only a minority of infected individuals develop clinical symptoms or diseases. The presence of virulence factors, such as CagA and VacA, has been associated with disease development, but assessment of virulence factor presence requires gastric biopsies. Here, we evaluate the Line test for risk stratification of infected patients by comparing the test score and immune recognition of type I or type II strains defined by the virulence factors CagA, VacA, GroEL, UreA, HcpC, and gGT with patient's disease status according to histology. Moreover, the immune responses of eradicated individuals from two different populations were analysed. Their immune response frequencies and intensities against all antigens except CagA declined below the detection limit. CagA was particularly long lasting in both independent populations. An isolated CagA band often represents past eradication with a likelihood of 88.7%. In addition, a high Line score was significantly associated with high-grade gastritis, atrophy, intestinal metaplasia, and gastric cancer. Thus, the Line is a sensitive and specific noninvasive test for detecting serum responses against in actively infected and eradicated individuals. Moreover, it allows stratifying patients according to their disease state.
感染显示出全球约 50%的患病率。然而，只有少数感染者会出现临床症状或疾病。毒力因子的存在，如 CagA 和 VacA，与疾病的发展有关，但毒力因子存在的评估需要胃活检。在这里，我们通过比较 Line 试验评分和对 I 型或 II 型菌株的免疫识别来评估感染患者的风险分层，这些菌株由 CagA、VacA、GroEL、UreA、HcpC 和 gGT 等毒力因子定义，并根据组织学确定患者的疾病状态。此外，还分析了来自两个不同人群的已根除个体的免疫反应。他们对除 CagA 以外的所有抗原的免疫反应频率和强度均下降到检测限以下。CagA 在两个独立的人群中都特别持久。单独的 CagA 带通常代表过去的根除，可能性为 88.7%。此外，高 Line 评分与高级别胃炎、萎缩、肠化生和胃癌显著相关。因此，Line 是一种敏感和特异性的非侵入性检测方法，可用于检测活跃感染和根除个体对血清的反应。此外，它还可以根据患者的疾病状态对其进行分层。
