Liu Lingqi, Li Yanqin, Liu Shuchao, Duan Qixin, Chen Liang, Wu Tianpeng, Qian Huijun, Yang Sixing, Xin Dianqi
1 Department of Urology, Renmin Hospital of Wuhan University, Wuhan, China.
2 School of Pharmaceutical Sciences, Wuhan University, Wuhan, China.
Tumour Biol. 2017 Jun;39(6):1010428317711951. doi: 10.1177/1010428317711951.
Although miR-193a-3p has been found to be dysregulated in variety of human tumors, little is known about its role in renal cell carcinoma. This study was designed to investigate the function and underlying mechanism of miR-193a-3p in human renal cell carcinoma tissues and cell lines. Here, we demonstrated that the expression of miR-193-3p was increased in renal cell carcinoma tissues and cell lines. In addition, knockdown of miR-193a-3p significantly inhibited cell proliferation and colony formation and induced cells into G1 phase arrest. Meanwhile, the migration potential of 786-O cells was also decreased compared to control group. Furthermore, we identified PTEN as a direct and functional target of miR-193a-3p, at least partly responsible for promoting tumor effect of miR-193a-3p in renal cell carcinoma. Taken together, the findings indicated for the first time that miR-193a-3p functions as a tumor-promoting microRNA by directly targeting PTEN in renal cell carcinoma.
尽管已发现miR-193a-3p在多种人类肿瘤中表达失调，但其在肾细胞癌中的作用却知之甚少。本研究旨在探讨miR-193a-3p在人肾细胞癌组织和细胞系中的功能及潜在机制。在此，我们证明了miR-193-3p在肾细胞癌组织和细胞系中的表达增加。此外，敲低miR-193a-3p可显著抑制细胞增殖和集落形成，并诱导细胞进入G1期阻滞。同时，与对照组相比，786-O细胞的迁移能力也降低。此外，我们确定PTEN是miR-193a-3p的直接功能性靶点，至少部分负责miR-193a-3p在肾细胞癌中的促肿瘤作用。综上所述，这些发现首次表明miR-193a-3p在肾细胞癌中通过直接靶向PTEN发挥促肿瘤微小RNA的作用。
