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一种测定丙酮酸脱氢酶复合物和乙酰辅酶A合成酶的新放射化学方法。

A new radiochemical method for determination of pyruvate dehydrogenase complex and acetyl-coenzyme A synthetase.

作者信息

Liedvogel B

出版信息

Anal Biochem. 1985 Jul;148(1):182-9. doi: 10.1016/0003-2697(85)90643-8.

DOI:10.1016/0003-2697(85)90643-8
PMID:2864000
Abstract

A radiochemical method for assaying pyruvate dehydrogenase complex and acetyl-coenzyme A synthetase is described, using [2-14C]pyruvate and [1-14C]acetate, respectively, as radiolabeled precursors. The assay is based on nonenzymatic O-acylation of excess dithioerythritol (DTE) by enzymatically formed acetyl-coenzyme A. [1-14C]Acetyl-DTE is easily extracted from the incubation mixture by organic solvents and separated from the unreacted labeled substrates.

摘要

描述了一种用于测定丙酮酸脱氢酶复合物和乙酰辅酶A合成酶的放射化学方法，分别使用[2-¹⁴C]丙酮酸和[1-¹⁴C]乙酸盐作为放射性标记前体。该测定基于酶促形成的乙酰辅酶A对过量二硫苏糖醇（DTE）的非酶促O-酰化反应。[1-¹⁴C]乙酰-DTE可通过有机溶剂轻松从孵育混合物中提取出来，并与未反应的标记底物分离。

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Anal Biochem. 1985 Jul;148(1):182-9. doi: 10.1016/0003-2697(85)90643-8.
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