Tomasek Milan, Misak Anton, Grman Marian, Tomaskova Zuzana
MT Research, Bratislava, Slovak.
Institute of Clinical and Translational Research, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovak.
FEBS Lett. 2017 Aug;591(15):2251-2260. doi: 10.1002/1873-3468.12721. Epub 2017 Jul 12.
Recently, it has been discovered that isoforms of intracellular chloride channels (CLIC) are present in cardiac mitochondria. By reconstituting rat cardiac mitochondrial chloride channels into bilayer lipid membranes, we detected three equally separated subconductance states with conductance increment of 45 pS and < 2% occupancy. The observed rare events of channel decomposition into substates, accompanied by disrupted gating, provide an insight into channel quaternary structure. Our findings suggest that the observed channels work as four functionally coupled subunits with synchronized gating. We discuss the putative connection of channel activity from native mitochondria with the recombinant CLIC channels. However, conclusive evidence is needed to prove this connection.
最近，人们发现细胞内氯离子通道（CLIC）的亚型存在于心脏线粒体中。通过将大鼠心脏线粒体氯离子通道重组到双层脂质膜中，我们检测到三种等间距的亚电导状态，电导增量为45 pS，占据率小于2%。观察到的通道分解为亚状态的罕见事件，伴随着门控的破坏，为通道四级结构提供了深入了解。我们的研究结果表明，观察到的通道作为四个功能耦合的亚基起作用，门控同步。我们讨论了天然线粒体通道活性与重组CLIC通道之间的假定联系。然而，需要确凿的证据来证明这种联系。
